Biopolym. Cell. 1991; 7(4):20-26.
http://dx.doi.org/10.7124/bc.0002DB
Cell Biology
Genetic transformation of cereals: advances and perspectives
1Pasternak T. P.
  1. Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Methods for the genetic manipulation of cereal crops have progressed considerably in recent years. A serious limitations in the wider application of DNA-mediated direct gene transfer to cereal crops remains the difficulty of regenerating plants from isolated protoplasts. Alternative transformation methods independent of protoplasts are therefore being sought by introducing foreign genes either via microinjection into multicellular structure, or by delivering the isolated DNA directly into plants, or pollen transformation, or Agrobacterium mediated gene transfer. Most of these methods led to obtained genetically transformed cereal plants, but most suitable method in this case is delivering the isolated DNA directly into plants.
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References

[1] Hernalsteens JP, Thia-Toong L, Schell J, Van Montagu M. An Agrobacterium-transformed cell culture from the monocot Asparagus officinalis. EMBO J. 1984;3(13):3039-41.
[2] Hooykaas-Van Slogteren GMS, Hooykaas PJJ, Schilperoort RA. Expression of Ti plasmid genes in monocotyledonous plants infected with Agrobacterium tumefaciens. Nature. 1984;311(5988):763–4.
[3] Schäfer W, Görz A, Kahl G. T-DNA integration and expression in a monocot crop plant after induction of Agrobacterium. Nature. 1987;327(6122):529–32.
[4] Grimsley N, Hohn B, Ramos C, Kado C, Rogowsky P. DNA transfer from Agrobacterium to Zea mays or Brassica by agroinfection is dependent on bacterial virulence functions. Mol Gen Genet. 1989;217(2-3):309-16.
[5] Brettschneider R, Lasaeri PA, Lanqrige P. et al. Cereal transformation with Agrobaeterium tumefaciens via the pollen tube pathway. 7-th Int. congr. on plant tissue and cell culture: Abstrs. Amsterdam. 1990;49.
[6] Hensgens LA, Rueb S, Meijer EGM et al. Rice transformation, vectors for rice transformation and expression of transferred genes. 7-th Int. congr. on plant tissue and cell culture: Abstrs. Amsterdam. 1990:60.
[7] Potrykus I, Saul MW, Petruska J, Paszkowski J, Shillito RD. Direct gene transfer to cells of a graminaceous monocot. Molec Gen Genet. 1985;199(2):183–8.
[8] Lorz H, Baker B, Schell J. Gene transfer to cereal cells mediated by protoplast transformation. Molec Gen Genet. 1985;199(2):178–82.
[9] Rhodes CA, Pierce DA, Mettler IJ, Mascarenhas D, Detmer JJ. Genetically transformed maize plants from protoplasts. Science. 1988;240(4849):204-7.
[10] Terada R, Shimamoto K. Expression of CaMV35S-GUS gene in transgenic rice plants. Mol Gen Genet. 1990;220(3):389–92.
[11] Vasil LK, Vasil V, Redway F. Plant regeneration from embryogenic protoplasts of Triticum aestivum L. (wheat). 7-th Int. congr. on plant tissue and cell culture. Abstrs. Amsterdam. 1990:3.
[12] Tilton VR, Russell SH. Microinjection of plant cells. II. Cell isolation, preparation and initial culture. Plant Physiol. 1983; 12(1):9.
[13] Pasternak TP, Melnikov PV. Genetic transformation of higher plants using DNA microinjection. Biology of cultivated cells and biotechnology: Proceedings Intern. Conference Novosibirsk, 1988:187-188.
[14] Neuhaus G, Spangenberg G, Mittelsten Scheid O, Schweiger H-G. Transgenic rapeseed plants obtained by the microinjection of DNA into microspore-derived embryoids. Theor Appl Genet. 1987;75(1):30-6
[15] Wei ZM, Kyo M, Harada H. Callus formation and plant regeneration through direct culture of isolated pollen of Hordeum vulgare cv. 'Sabarlis'. Theor Appl Genet. 1986;72(2):252-5.
[16] Kartha KK, Chibbar RN, Georges F, Leung N, Caswell K, Kendall E, Qureshi J. Transient expression of chloramphenicol acetyltransferase (CAT) gene in barley cell cultures and immature embryos through microprojectile bombardment. Plant Cell Rep. 1989;8(8):429-32.
[17] Vallejos RH, Lopez M, Lorsaria L. et al. Plant cell transformation using high velocity microparticles. 7-th Int. cong. on plant tissue and cell culture: Abstrs. Amsterdam. 1990:80.
[18] Chen D, Batty N, Evans J, Dale P. Transformation of cereal cell cultures by microprojective bombardment. 7-th Int. cong. on plant tissue and cell culture: Abstrs. Amsterdam. 1990:50.
[19] Mendel RR, Claus E, Schulze J. et al. Gene transfer to barley. 7-th Int. cong. on plant tissue and cell culture: Abstrs. Amsterdam. 1990:44.
[20] Kartel NA. Effects of exogenous DNA in higher plants. Minsk: Nauka i tekhnika, 1981; 143 p.
[21] Ledoux L, Diels L, Huart R. et al. Transfer of bacterial and human genes to germinating Arabidopsis thaliana. Arabidopsis inform, service. 1985; 22: 1-12.
[22] Töpfer R, Gronenborn B, Schell J, Steinbiss HH. Uptake and transient expression of chimeric genes in seed-derived embryos. Plant Cell. 1989;1(1):133-9.
[23] De la Peña A, Lörz H, Schell J. Transgenic rye plants obtained by injecting DNA into young floral tillers. Nature. 1987;325(6101):274–6.
[24] Hess D. Pollen-based techniques in genetic manipulation. Int Rev Cyt. Volume 107 1987;367–95.
[25] De Wet JM, Bergqust R, Harlan J. Exogenous gene transfer in maize using DNAtreated pollen. Exp. manipulation of ovule tissues. Ed. P. Charman. New York, 1985:197-209.
[26] Ohta Y. High-efficiency genetic transformation of maize by a mixture of pollen and exogenous DNA. Proc Natl Acad Sci U S A. 1986;83(3):715-9.
[27] Chesnokov YuV, Vikonskaya NA, Korol' AB. Genetic transformation of maize. 7th Proc. symposium. "Molecular mechanisms of genetic processes": Abstracts M. 1990:183.
[28] Booy G, Krens FA, Huizing HJ. Attempted Pollen-Mediated Transformation of Maize. J Plant Physiol. 1989;135(3):319–24.
[29] Luo Z, Wu R. A simple method for the transformation of rice via the pollen-tube pathway. Plant Mol Biol Rep. 1988;6(3):165–74.
[30] Kartel' NA, Zaben'kova KI, Maneshina TV, Ablov SE.Barley plants with the gene-introduced kanamycin resistant. Dokl Akad Nauk BelSSSR. 1990; 34(3):261-3.
[31] Flavell R, Mathias R. Genetic engineering: Prospects for transforming monocot crop plants. Nature. 1984;307(5947):108–9.
[32] Pasternak TP. Introduction of foreign genes into plant cereals. 11th All-Union Congress of All-Union Society of Plant Physiologists: Proc. of reports. Minsk, 1990:72.