Biopolym. Cell. 1991; 7(4):6-20.
http://dx.doi.org/10.7124/bc.0002DA
Cell Biology
Individual culturing of preselected plant protoplasts and their fusion products in microdroplets
1Kirichenko I. V., 1Gleba Yu. Yu.
  1. Institute of Cell Biology and Genetic Engineering, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Successfull development of different plant species single cells, protoplasts and products of preselected single protoplast fusion has been shown using the method of individual culturing in microdroplets developed. The highest plating efficiency among the individual protoplasts reached about 75% (soybean), the lowest one – 2,5% (clover). Electrofusion efficiency was as high as 95%. In this article we describe our individual culturing and electrofusion technology.
Full text: (PDF, in Russian)

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