Biopolym. Cell. 1991; 7(3):35-38.
http://dx.doi.org/10.7124/bc.0002CC
Obtaining of the anti-sense cDNA construction of human low-density lipoprotein receptor
1Dolzhanskaya M. T., 1Todorova M. T., 1Schwarzman A. L.
  1. Institute of Experimental Medicine, Academy of Medical Sciences of the USSR
    Leningrad, USSR

Abstract

The anti-sense cDNA construction of human low-density lipoprotein receptor (rLDL) was obtained. It includes the 5'-terminal fragment of cDNA rLDL and the murine metal-lothionein gene promoter. The construction may be used to obtain transgenic animals that are deficient of rLDL.
Full text: (PDF, in Russian)

References

[1] Green PJ, Pines O, Inouye M. The role of antisense RNA in gene regulation. Annu Rev Biochem. 1986;55:569-97.
[2] Brown MS, Goldstein JL. A receptor-mediated pathway for cholesterol homeostasis. Science. 1986;232(4746):34-47.
[3] Palmiter RD, Norstedt G, Gelinas RE, Hammer RE, Brinster RL. Metallothionein-human GH fusion genes stimulate growth of mice. Science. 1983;222(4625):809-14.
[4] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
[5] Calvin NM, Hanawalt PC. High-efficiency transformation of bacterial cells by electroporation. J Bacteriol. 1988;170(6):2796-801.
[6] Hammer RE, Brinster RL, Rosenfeld MG, Evans RM, Mayo KE. Expression of human growth hormone-releasing factor in transgenic mice results in increased somatic growth. Nature. 1985 May 30-Jun 5;315(6018):413-6.
[7] Dolzhanskaya NB, Mandelshtam MYu, Patkin EL, Kuznetsov AS, Vikhanskaya FL, Krutilina RI, Gaitskhoki VS. A model for genetic correction of family hypercholesterolemia. Biopolym Cell. 1990; 6(2):31-5.