Biopolym. Cell. 1991; 7(2):103-109.
http://dx.doi.org/10.7124/bc.0002C6
Discussions
Age peculiarities of human apoAl gene implantation effect on the development of dislipoproteidemias in the experimental animals
1Frolkis V. V., 2Kordium V. A., 2Bogatskaya L. N., 2Novikova S. N., 2Shulzhenko V. N., 2Kostetsky I. E., 1Mozzhukhina T. G., 1Potapenko R. I., 1Sabko V. E., 1Paramonova G. I.
  1. Gerontology Institute of the Academy of Medical Sciences of Ukraine
    67, Vyshgorodska Str., Kyiv, Ukraine, 04114
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

After introduction of human apo Al gene as in an expression vector into liver of adult and old rats we could reveal this human protein in the serum of the animals. Changes in HDL, HDL3 and HDL2 subfamilies its protein and lipid contents could be observed also. A higher apo Al gene expression was observed in adult young animals while old animals, showed greater differences in protein and lipid contents.
Full text: (PDF, in Russian)

References

[1] Repin VS. Human Atherosclerosis: cellular and molecular mechanisms. Usp Sovrem Biol. 1990; 109(1):120-9.
[2] Frolkis VV. Aging. Neurohumoral mechanisms. Kiev: Naukova Dumka, 1981; 320 p.
[3] Korpushko OV, Bogatskaya LN, Novikov SN et al. Dyslipidemia in older adults with major clinical forms of atherosclerosis. Dyslipidemia in atherosclerosis in the elderly people. Kyiv, 1985; Iss. 7:11-32.
[4] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
[5] Shulzhenko VN, Lukash LL, Shulyak LN, Usenko EV, Kordyum VA. Cloning of human ApoA1 gene and its expression in murine fibroblasts. Biopolym Cell. 1989;5(5):105-7.
[6] Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499-560.
[7] Margolis LB, Bergelson LD Liposomes and their interaction with the cells. Moscow: Nauka, 1986. 240 p.
[8] Gaal Ö, Medgyesi G A, Vereczkey L. Electrophoresis in the separation of biological macromolecules. John Wiley & Sons, Chichester, UK and Akademiai Kiado, Budapest. 1980; 422 p.
[9] Klimov AN, Usatenko MS, Denisenko AD, Kozhevnikova KA, Oleĩnik IA. Isolation of apolipoproteins A-I, A-II and E and their estimation by rocket immunoelectrophoresis in blood plasma of dis-alpha-lipoproteinemic patients. Biokhimiia. 1981;46(4):590-602.
[10] Markwell MA, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978;87(1):206-10.
[11] Kates M. Techniques of lipidology: isolation, analysis and identification of lipids. North-Holland Publishing Company, 1975; 269 p.
[12] Svanborg A, Svennerholm L, Myrén I, Soomägi MR, Claesson B, Rohman S. Plasma total lipid, cholesterol, triglycerides, phospholipids and free fatty acids in a healthy scandinavian population. Acta Med Scand. 1961;169(1):43–9.
[13] Swaney JB, Kuehl KS. Separation of apolipoproteins by an acrylamide-gradient sodium dodecyl sulfate gel electrophoresis system. Biochim Biophys Acta. 1976;446(2):561-5.
[14] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.
[15] Karuzina II, Archakov AI. Isolation of liver microsomal fractions and characterization of its oxidation systems. Modern methods in biochemistry. M.: Medicine, 1977: 49-62.
[16] Omura T, Sato R. The carbon monoxide-binding pigment of liver microsomes. II. Solubilization, purification, and properties. J Biol Chem. 1964;239:2379-85.
[17] Biriukova RN. On the problem of determination of the mean square root derivation on the basis of amplitude. Gig Sanit. 1962;27:43-6.
[18] Shulhzenko VN, Novikova SN, Kostetsky IE, Bogatskaya LN, Frolhis VV, Kordium VA. Transfer of cloned human gene ApoA1 into adult and old rabbits. Biopolym Cell. 1990; 6(2):71-6.