Biopolym. Cell. 1991; 7(2):80-86.
http://dx.doi.org/10.7124/bc.0002C2
Discussions
Cloning of cDNA encoding bovine follicle-stimulating hormone
1Gorbulev V. G., 1Chernov B. K., 1Rubtsov P. M.
  1. Engelhardt Institute of Molecular Biology, Academy of Sciences of the USSR
    Moscow, USSR

Abstract

The λgt10 library of bovine pituitary cDNA was screened and the clones with cDNA encoding α subunit of glycoprotein hormones were isolated. The sequencing of the inserts from two clones revealed that they containe 3'-terminal nucleotide of mRNA followed by the prolonged (about 300 bp) but different stretch. The clones carrying the cDNA for β subunit of follicle-stimulating hormone (FSH) were isolated from the enriched plasmid library of bovine pituitary cDNA. The sequence of cDNA for β subunit of FSH was established. The sequence results proved that we have cDNAs encoding the full-length both a and β subunits of bovine FSH.
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