Biopolym. Cell. 1991; 7(2):22-24.
http://dx.doi.org/10.7124/bc.0002BF
Gene-Engineering Biotechnology
The influence of UV irradiation on the transfer of foreign genes into Ti plasmid of Agrobacterium tumefaciens via intermediate vectors
1Dobachevskaya O. N., 1Rymar S. E., 1Kordium V. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The influence of UV irradiation of recipient A. tumefaciens cells on the frequency of the foreign gene transfer into Ti plasmid via intermediate vectors has been investigated. It has been established that the frequency of this process after transient irradiation of recipient cells is 10–100 times higher than the frequency observed without irradiation. This fact is suggested to be used as an effective method to increase the foreign gene transfer frequency into Ti plasmid if the intermediate vectors are applied for it.
Full text: (PDF, in Russian)

References

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