Biopolym. Cell. 1991; 7(2):19-22.
Gene-Engineering Biotechnology
Optimization of the β-lactamase TEM1 phage-dependent synthesis system in Escherichia coli cells
1Sorochinskaya T. V., 1Chernykh S. I., 1Kordium V. A.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
The original system of the β-lactamase gene amplification has been worked out. Such a system can produce 6.0·105 units of β-lactamase activity per ml of cultural medium and has such an advantage as the accessible enzyme form.