Biopolym. Cell. 1991; 7(2):8-15.
http://dx.doi.org/10.7124/bc.0002BC
Structure and Function of Biopolymers
Fractionation of eukaryotic DNA by pulsed field gel electrophoresis. Enzymatic nature of the formation
1Solovyan V. T., 1Makytryuck V. L., 1Rudenko G. N.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. Institute of Cell Biology and Genetic Engineering, NAS of Ukraine
    148, Akademika Zabolotnogo Str., Kyiv, Ukraine, 03680

Abstract

The data presented here confirm the possibility of enzymatic formation of discrete DNA-fragments, appearing during the fractionation of lysed nuclei by FIGE. Teniposide-dependent pattern of DNA-fragments and occurrence of protein-linked DNA breaks suggest that discrete cleavage of intact nuclear DNA is modulated by DNA-topoisomerase II. It is pointed that pulsed field gel electrophoresis may be a useful method to study higer-order chromatin structure.
Full text: (PDF, in Russian)

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