Biopolym. Cell. 1991; 7(1):101-107.
http://dx.doi.org/10.7124/bc.0002BA
Gene-Engineering Biotechnology
Study of β-lactamase tem1 synthesis in various escherichia coli strains infected by the phage λ bla with amber-mutations in different regulatory genes
1Sorochynskaja T. V., 1Chernykh S. I.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The search of optimal conditions for phage-dependent supersynthesis of β-lactamase was undertaken. For this purpose the recombinant phage with alternatively orientated β-lactamase (bla) structure gene were obtained. It was stated, that phage λblaN–N-, λblaQ–R– derivates are obtained. The β-lactamase producing strain was selected. The maximal recovery of β-lactamase was obtained while Su0 E. coli W3101 strain was infected by λ-phage bla Q–R–.
Full text: (PDF, in Russian)

References

[1] Glushakova LG, Chernykh SI, Stelmashenko LN, Kordium VA. beta-galactosidase supersynthesis indiced by some amber mutation of phage lambda plac3Cl857. Molekulyarnaya biologiya (Kiev). 1982;30:37-41.
[2] Glushakova LG, Chernykh SI, Kordium VA. Functioning of N-derivatives of phage plac3Cl857 in E. coli Suo. Dokl Akad Nauk SSSR. 1982; 267(12):56-9.
[3] Kordium VA, Glushakova LG, Chernykh SI. Use of N-derivatives of lambda plac 5 phage for the purpose of beta-galactosidase supersynthesis. Dokl Akad Nauk SSSR. 1980;255(3):760-3.
[4] Davison J, Brammar WJ, Brunel F. Quantitative aspects of gene expression in a lambda-trp fusion operon. Mol Gen Genet. 1974;130(1):9-20.
[5] Murray NE, Kelley WS. Characterization of lambdapolA transducing phages; effective expression of the E. coli polA gene. Mol Gen Genet. 1979;175(1):77-87.
[6] Clewell DB, Helinski DR. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969;62(4):1159-66.
[7] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
[8] Dagert M, Ehrlich SD. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979;6(1):23-8.
[9] Miller JH. Experiments in molecular genetics. Cold Spring Harbor Laboratory, 1972, 466 p.
[10] Chaikovskaia SM, Venkina TG. A modified iodometric method for the determination of penicillinase activity. Antibiotiki. 1962;7:453-6.
[11] Gottesman ME, Adhya S, Das A. Transcription antitermination by bacteriophage lambda N gene product. J Mol Biol. 1980;140(1):57-75.
[12] Honigman A. Cloning and characterization of a transcription termination signal in bacteriophage lambda unresponsive to the N gene product. Gene. 1981;13(3):299-309.
[13] Dambly-ChaudiГЁre C, Gottesman M, Debouck C, Adhya S. Regulation of the pR operon of bacteriophage lambda. J Mol Appl Genet. 1983;2(1):45-56.
[14] Drahos D, Szybalski W. Antitermination and termination functions of the cloned nutL, N, and tL1 modules of coliphage lambda. Gene. 1981;16(1-3):261-74.
[15] Salstrom JS, Szybalski W. Coliphage lambdanutL-: a unique class of mutants defective in the site of gene N product utilization for antitermination of leftward transcription. J Mol Biol. 1978;124(1):195-221.
[16] The Bacteriophage lambda. Ed. A. D. Hershey. N. Y. Cold Spring Harbor Lab, 1971. NLM ID: 0313535
[17] Kleckner N, Signer ER. Genetic characterization of plasmid formation by N- mutants of bacteriophage lambda. Virology. 1977;79(1):160-73.
[18] Herskowitz I, Signer ER. A site essential for expression of all late genes in bacteriophage lambda. J Mol Biol. 1970;47(3):545-56.
[19] Grayhack EJ, Roberts JW. The phage lambda Q gene product: activity of a transcription antiterminator in vitro. Cell. 1982;30(2):637-48.
[20] Dambly C, Delstanche M, Gathoye AM. qin101: Promoter mutation which allows the constitutive expression of the late genes. J Virol. 1979;30(1):14-20.
[21] Bienkowska-Szewczyk K, Lipinska B, Taylor A. The R gene product of bacteriophage lambda is the murein transglycosylase. Mol Gen Genet. 1981;184(1):111-4.
[22] Moir A, Brammar WJ. The use of specialised transducing phages in the amplification of enzyme production. Mol Gen Genet. 1976;149(1):87-99.
[23] Lieb M. Lambda mutants which persist as plasmids. J Virol. 1970;6(2):218-25.