Biopolym. Cell. 1991; 7(1):63-69.
http://dx.doi.org/10.7124/bc.0002B3
Structure and Function of Biopolymers
High molecular weight complex of tyrosyl-tRNA synthetase from bovine liver
1Gnatenko D. V., 1Kornelyuk A. I., 1Kurochkin I. V., 1Matsuka G. H.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

The tyrosyl-tRNA synthetase in postribosomal bovine liver supernatant is included as a compound of the high molecular weight complex with Mr > 106 Da. Dissociation of tyrosyl-tRNA synthetase from this complex due to proteolysis proceeds through the intermediate labile complex with Mr≈240 kDa. The thermostability of tyrosyl-tRNA synthetase at this intermediate labile complex is much higher in comparison with free enzyme. The labile intermediate complex of bovine liver tyrosyl-tRNA synthetase possesses the nonspecific affinity for high molecular weight ribosomal RNA.
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