Biopolym. Cell. 1990; 6(5):86-90.
http://dx.doi.org/10.7124/bc.000293
Cell Biology
Fertile intertribal symmetrical somatic hybrids in the solanaceae
1Babiychuk E. L., 1Kushnir S. G., 1Gleba Yu. Yu.
  1. Department of Cell Biology and Engineering, N. G. Kholodny Institute of Botany, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Usage of the genetically homogeneous, euploid mesophyll protoplasts and strict genetical selection is supposed as particularly important rule for production of the normal remote somatic hybrids. As a result the performed experiments, morphologically normal hybrids Nicotiana tabacum + Atropa belladonna, Nicotiana tabacum + Physochlalne officinalis possessing symmetrical quantities of the nuclear genetic material from both parents are obtained, that follows from the carried out cytogenetical and biochemical analyses. Sexual progeny was obtained after pollination of N. tabacum + A. belladonna hybrids with tobacco pollen. Fl plants possess 20-30 chromosomes of A. belladonna (2rc=72) and 48 chromosomes of N. tabacum (2«=48). Some important problems of genetics, physiology, developmental biology and genetical control of the secondary metabolite boisynthesis could be studied using described hybrids and their progeny. As is generally known described symmetrical somatic hybrids are the first fertile plants combining full nuclear genomes of the plants from different tribes.
Full text: (PDF, in Russian)

References

[1] Gleba YuYu, Sytnik KM. Cell engineering of plants. Kyiv, Naukova dumka, 1984; 160 p.
[2] Gleba Yu., Sytnik K. Protoplast fusion. Berlin: Springer, 1984. 203 p.
[3] Gleba YY, Hoffmann F. "Arabidobrassica": A novel plant obtained by protoplast fusion. Planta. 1980;149(2):112-7.
[4] Kushnir SG, Shlumukov LR, Pogrebnyak NJ, Berger S, Gleba Y. Functional cybrid plants possessing a Nicotiana genome and an Atropa plastome. Mol Gen Genet. 1987;209(1):159-63.
[5] Gleba YuYu, Kaleda VA, Parokonnyi AS, Borisyak NE, Lopato SV, Negrutsu I, Nanobs M. Fertile nuclear intertribe hybrids Nicotiana + Atropa, obtained by fusion of normal and inactivated by irradiation somatic cells. Dokl Akad Nauk SSSR. 1987; 297(6):1473-5.
[6] Dudits D, Maroy E, Praznovszky T, Olah Z, Gyorgyey J, Cella R. Transfer of resistance traits from carrot into tobacco by asymmetric somatic hybridization: Regeneration of fertile plants. Proc Natl Acad Sci U S A. 1987;84(23):8434-8.
[7] Guri A, Levi A, Sink KC. Morphological and molecular characterization of somatic hybrid plants between Lycopersicon esculentum and Solanum nigrum. Mol Gen Genet. 1988;212(2):191–8.
[8] Gleba YY, Momot VP, Cherep NN, Skarzynskaya MV. Intertribal hybrid cell lines of Atropa belladonna (X) Nicotiana chinensis obtained by cloning individual protoplast fusion products. Theor Appl Genet. 1982;62(1):75-9.
[9] Svab Z, Maliga P. Nicotiana tabacum mutants with chloroplast encoded streptomycin resistance and pigment deficiency. Theor Appl Genet. 1986;72(5):637-43.
[10] Maliga P, Sz-Breznovits A, Marton L Joo F. Non-Mendelian streptomycin-resistant tobacco mutant with altered chlorplasts and mitochondria. Nature. 1975;255(5507):401-2.
[11] Menczel L, Nagy F, Kiss ZR, Maliga P. Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids. Theor Appl Genet. 1981;59(3):191-5.
[12] Kao KN, Michayluk MR. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta. 1975;126(2):105-10.
[13] Caboche M. Nutritional requirements of protoplast-derived, haploid tobacco cells grown at low cell densities in liquid medium. Planta. 1980;149(1):7-18.
[14] Murashige T, Skoog F. A Revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473–97.
[15] An G, Watson BD, Stachel S, Gordon MP, Nester EW. New cloning vehicles for transformation of higher plants. EMBO J. 1985;4(2):277-84.