Quantitative determination of indole alkaloids in Rauwolfia cultured cells by microcolumn chromatography

Gubar, S. I.; Konstantinova, E. P.; Kunakh, V. A.

doi:10.7124/bc.000291

Biopolym. Cell. 1990; 6(5):78-80.

http://dx.doi.org/10.7124/bc.000291

Structure and Function of Biopolymers

Quantitative determination of indole alkaloids in Rauwolfia cultured cells by microcolumn chromatography

1Gubar S. I., 1Konstantinova E. P., 1Kunakh V. A.

Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
Kiev, USSR

Abstract

The separation conditions and method of indole alkaloid analysis on silica gel using gradient elution were elaborated by microcolumn chromatograph «Milichrom». The developed method permits carrying out simultaneous quantitative determination of ajmalicine, reserpine, vomilenine, ajmaline and serpentine in the tissue culture extract. Various Rauwolfia cell lines have been analyzed. About 30 different alkaloids were detected and contents of ajmalicine, reserpine, vomilenine, ajmaline and serpentine were measured in the cultured cells. The method requires a small amount of material to be analyzed (1 μl of 0.5% alkaloid solution for one measurement) and it is characterized by high precision and high rate of analysis.

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