Biopolym. Cell. 1990; 6(5):72-77.
http://dx.doi.org/10.7124/bc.000290
Structure and Function of Biopolymers
Immunoelectron-microscopic study of seryl-tRNA-synthetase localization in cells of higher eucaryotes
1Sidorik L. L., 2Popenko V. I., 2Cherny N. E., 1Tukalo M. A., 2Beresten S. F., 1Matsuka G. Kh.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR
  2. Engelhardt Institute of Molecular Biology, Academy of Sciences of the USSR
    Moscow, USSR

Abstract

Localization of seryl-tRNA-synthetase (SerRS) was studied on ultrathin (UT) section of bovine kidney cells fixed with glutaraldehyde and embedded in «Lowycril K4M» resin at – 35 °C. The UT sections were treated with the polyclonal affined-purified antibodies against SerRS and the complexes of protein A with colloidal gold 20 nm in size. In the cells the cytoplasm was the most intensely labelled. A great amount of SerRS was found in the cell nuclei: the label was concentrated over the diffuse chromatin localization regions, a minimal binding being observed over compact chromatin. The presence of SerRS in the eucaryotic cell nucleus may evidence for the possible regulatory role of SerRS in eucaryotes.
Full text: (PDF, in Russian)

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