Biopolym. Cell. 1990; 6(4):97-101.
http://dx.doi.org/10.7124/bc.000285
Gene-Engineering Biotechnology
A method for selection of hybridomas, secreting monoclonal antibodies against tyrosyl-tRNA synthetase, based on monitoring of the enzymatic activity
1Ribkinska T. A., 2Vartanyan O. A., 1Filonenko V. V., 1Sidorik L. L., 1Kornelyuk A. I., 2Beresten S. F.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR
  2. Engelhardt Institute of Molecular Biology, Academy of Sciences of the USSR
    Moscow, USSR

Abstract

A new method for screening populations of hybrid cells secreting monoclonal antibodies (Mabs) against aminnacyl-tRNA synthetases (ARS) is developed as based on monitoring of the enzymatic activity of ARSes. The main advantage of this procedure stems from the fact that it permits using non-homogeneous ARS preparations both for immunization and for screening. While applying this procedure, hybridoma cell line T3 secreting Mabs against bovine tyrosyl-tRNA synthetase has been produced and characterized.
Full text: (PDF, in Russian)

References

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