Biopolym. Cell. 1990; 6(4):92-96.
http://dx.doi.org/10.7124/bc.000284
Gene-Engineering Biotechnology
Expression in Escherichia coli of the human angiogenin gene translationally fused with the N-terminal fragment of β-galactosidase
1, 2Kovalenko S. P., 1Mertvetsov N. P.
  1. Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR
  2. Institute of Therapy, Siberian Branch of Medical Sciences of the USSR
    Novosibirsk, USSR

Abstract

The phage M13mp8 earring a DNA fragment coding for fusion protein angiogenin-α-peptide of β-galactosidase was obtained by oligonucleotide directed mutagenesis technique from the M13mp8 phage with a cloned synthetic human angiogenin gene. The synthesis of peptide with, α-donor activity in the phage Ml-1-infected cells was demonstrated. The amount of the fusion protein was estimated at the different stages of E. coll cell growth after the phage infection.
Full text: (PDF, in Russian)

References

[1] Löwenadler B, Jansson B, Paleus S, Holmgren E, Nilsson B, Moks T, Palm G, Josephson S, Philipson L, Uhlén M. A gene fusion system for generating antibodies against short peptides. Gene. 1987;58(1):87-97.
[2] Struck DK, Maratea D, Young R. Purification of hybrid beta-galactosidase proteins encoded by phi X174 E phi lacZ and Escherichia coli prlA phi lacZ: a general method for the isolation of lacZ fusion polypeptides produced in low amounts. J Mol Appl Genet. 1985;3(1):18-25.
[3] Paton EB, Zhyvoloup AN. Chimeric rpoC'- lacZ' gene of the recombinant pUC19 plasmid reserving the β-galactosidase activity in Escherichia coli cells. Biopolym. Cell. 1987; 3(5):276-9.
[4] Petrenko VA, Tat'kov SI, Semenova LN, Il'ichev AA, Pozdniakov PI. Expression in Escherichia coli of the human leukocyte interferon alpha2 gene fused with N-terminal fragment of beta-galactosidase. Mol Gen Mikrobiol Virusol. 1988;(4):37-41.
[5] Close TJ, Christmann JL, Rodriguez RL. M13 bacteriophage and pUC plasmids containing DNA inserts but still capable of beta-galactosidase alpha-complementation. Gene. 1983;23(2):131-6.
[6] Langley KE, Villarejo MR, Fowler AV, Zamenhof PJ, Zabin I. Molecular basis of beta-galactosidase alpha-complementation. Proc Natl Acad Sci U S A. 1975;72(4):1254-7.
[7] Kovalenko SP, Gorn VV, Karginov VA, Morozov IV, Zarytova VF. Chemico-enzymatic synthesis and cloning of human angiogenin gene in M13mp8 phage. Bioorg Khim. 1988;14(7):910-5.
[8] Landon. Cleavage at aspartyl-prolyl bonds. Methods Enzymol. 1977;47:145-9.
[9] EMBO. EMBL summer course on site directed mutagenesis: Lab. manual. Heidelberg, 1984. 59 p.
[10] Sigma Company Chemical Catalog. 1989. 631 p.
[11] Miller JH. Experiments in molecular genetics. Cold Spring Harbor Laboratory, 1972, 466 p.