Biopolym. Cell. 1990; 6(4):65-69.
http://dx.doi.org/10.7124/bc.00027F
Structure and Function of Biopolymers
Accessibility of ribosomal proteins to trypsin in Thermus thermophilus ribosomes
1Yusupov M. M., 2Spirina T. N.
  1. Institute of Protein Research, Academy of Sciences of the USSR
    Pushchino, Moscow Region, USSR
  2. Institute of Molecular Genetics, Academy of Sciences of the USSR
    Moscow, USSR

Abstract

Proteins accessible to trypsin in ribosome and in ribosomal subunits from extreme thermophilic Thermus thermophilus have been analyzed. In 70S ribosomes 7 proteins of the small (SOS) subunit, TS2, TS3, TS7, TS12, TS17, TS19, TS20 and 5 proteins of the large (SOS) subunit, TL5, TL8, TL9, TL23, TL24 were accessible to trypsin. Dissociation of 70S ribosomes into subunits leads to no appearance of proteins accessible to trypsin. This confirms previous conclusion on the absence of ribosomal proteins between the subunits (Yusupov M. and Spirin A., FEES Lett., 1986, 197, 229—233).
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References

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