Biopolym. Cell. 1990; 6(3):90-94.
http://dx.doi.org/10.7124/bc.000274
Gene-Engineering Biotechnology
Pulsed field gradient electrophoresis study of pYF91 plasmid integration into yeast chromosomes
1Mezhevaya E. V., 2Stepanova V. P., 1Yarovoy B. F., 1Beritashvili D. R., 3Zakharov I. A.
  1. B. P. Konstantinov Institute of Nuclear Physics, Academy of Sciences of the USSR
    Gatchina, Leningrad distr., USSR
  2. Institute of Molecular Biology, Academy of Sciences of the USSR
    Moscow, USSR
  3. N. I. Vavilov Institute of General Genetics, Academy of Sciences of the USSR
    Moscow, USSR

Abstract

An electrophoretic karyotyping of yeast strains with the integrated plasmid was performed. The collection used was comprised of strains with plasmid pYFSl integrated in several chromosomes, namely I, III, VI, IX, XI, respectively. Some bands positions for integrants were clearly different from those of the recipient strain. In each case only the chimaeric chromosome position changed. Thus, it is shown possible to identify chimaeric chromosomes by the technique of pulsed field gradient gel electrophoresis.
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