Biopolym. Cell. 1990; 6(3):81-84.
http://dx.doi.org/10.7124/bc.000272
Gene-Engineering Biotechnology
Cloning of the Malakosoma neustria nuclear polyhedrosis virus polyhedrin gene
1Strokovskaya L. I., 1Veselovsky O. V., 1Kikhno I. M., 1Mirjuta N. Yu.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

This investigation is the first step of baculovirus expressive vector construction using the Malakosoma neustria nuclear polyhedrosis virus (MnNPV). The polyhedrin gene has been localized in the MnNPV genome. Recombinant plasmid has been constructed carryinga viral DNA fragment containing a polyhedrin gene with flanking DNA nucleotide sequences. This plasmid can be used for the transfer vector construction.
Full text: (PDF, in Russian)

References

[1] Luckow VA, Summers MD. Trends in the Development of Baculovirus Expression Vectors. Bio/Technology.1988;6(1):47–55.
[2] Vlak JM, Klinkenberg FA, Zaal KJ, Usmany M, Klinge-Roode EC, Geervliet JB, Roosien J, van Lent JW. Functional studies on the p10 gene of Autographa californica nuclear polyhedrosis virus using a recombinant expressing a p10-beta-galactosidase fusion gene. J Gen Virol. 1988;69 ( Pt 4):765-76.
[3] Smith GE, Summers MD, Fraser MJ. Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol Cell Biol. 1983;3(12):2156-65.
[4] Maeda S, Kawai T, Obinata M, Fujiwara H, Horiuchi T, Saeki Y, Sato Y, Furusawa M. Production of human alpha-interferon in silkworm using a baculovirus vector. Nature. 1985 Jun 13-19;315(6020):592-4.
[5] Kok IP, Skuratovskaya IP, Strokovskaya LI. Molecular basis of reproduction baculovirus. Kyiv, Naukova Dumka, 1980; 175 p.
[6] Skuratovskaya IN, Strokovskaya LI, Komissarenko SV, Mendeleva NV. Structure and functioning of the nuclear polyhedrosis virus genome in Melacosoma neustria. Tsitol Genet. 1986; 20(1):31-6.
[7] Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab. press, 1982 545 p.
[8] Rohrmann GF, Leisy DJ, Chow KC, Pearson GD, Beaudreau GS. Identification, cloning, and R-loop mapping of the polyhedrin gene from the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata. Virology. 1982;121(1):51-60.
[9] Rohrmann GF. Polyhedrin structure. J Gen Virol. 1986;67 ( Pt 8):1499-513.
[10] Smith GE, Summers MD. DNA homology among subgroup A, B, and C baculoviruses. Virology. 1982;123(2):393-406.