Biopolym. Cell. 1990; 6(3):24-31.
http://dx.doi.org/10.7124/bc.000267
Structure and Function of Biopolymers
Low-frequency phonon Raman spectra of nucleic acid constituents: purine bases
1Govorun D. N., 1Mishchuk Ya. R., 1Zheltovsky N. V.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Low-frequency (10 cm-1 < v < 200 cm -1) Raman spectra of polycrystalline adenine and guanine at 300 K have been studied in detail. Lattice vibration bands are identified. The conclusions about the structural properties of the investigated substances are drawn after the consideration of theoretical and experimental data including those obtained in this work. Possible applications of the obtained results in the field of biophysics are discussed.
Full text: (PDF, in Russian)

References

[1] Shtepanek I, Baumruk V. Raman spectroscopy of nucleic acids components. Proc. of the 2nd coordination seminar of USSR-Czechoslovakia Ministry of Education. Vilnius, Mokslal, 1986; 34-42.
[2] Elinek O. Model systems of nucleic acids. Proc. of the 2nd coordination seminar of USSR-Czechoslovakia Ministry of Education. Vilnius, Mokslal, 1986; 84-91.
[3] Maleev VIa. Spiral oscillation of bases in nucleic acid. Biofizika. 1965;10(5):729-34. Russian.
[4] Urabe H, Tominaga Y, Kubota K et al. Low-lying collective vibrational modes in DNA double helix. Proc. 9 Int. conf. Raman spectr. Tokyo, 1984:158-159.
[5] Weidlich T, Lindsay SM. Raman study of the low frequency vibrations of polynucleotides. J Phys Chem. 1988;92(23):6479–82.
[6] Weidlich T, Lindsay SM, Lee SA, Tao NJ, Lewen GD, Peticolas WL, et al. Low-frequency Raman spectra of DNA: a comparison between two oligonucleotide crystals and highly crystalline films of calf thymus DNA. J Phys Chem. 1988;92(12):3315–7.
[7] Nielsen OF, Lund PA, Nielsen LS, Praestgaard E. Aspects of low frequency vibrations (20-350 cm-1) from Watson-Crick base pairing in an aqueous solution of tRNA. Biochem Biophys Res Commun. 1983;111(1):120-6.
[8] Nielsen OF, Chrystensen DH. Low frequency Raman spectra (20-200 cm-1) of nucleosides, nucleotides and nucleic acids in aqueous solutions. Spectroscopy of biological molecules. Chichester; New York: John Wiley Sons, 1985:127-9.
[9] Wittlin A, Genzel L, Kremer F, Häseler S, Poglitsch A, Rupprecht A. Far-infrared spectroscopy on oriented films of dry and hydrated DNA. Phys Rev A. 1986;34(1):493-500.
[10] Powell JW, Edwards GS, Genzel L, Kremer F, Wittlin A, Kubasek W, Peticolas W. Investigation of far-infrared vibrational modes in polynucleotides. Phys Rev A. 1987;35(9):3929-3939.
[11] Beetz CP Jr, Asearelli G. The low-frequency vibrational modes of an RNA: poly(I)-poly(C). Biopolymers. 1976;15(11):2299-301.
[12] Devi Prasad KV, Prohofsky EW. Low-frequency lattice mode predictions in A-DNA compared to experimental observations and significance for A-to-B conformation change. Biopolymers. 1984;23(9):1795-8.
[13] Devi Prasad KV, Prohofsky EW. Conformational changes in DNA and soft modes. J Biomol Struct Dyn. 1985;3(3):551-8.
[14] Webb SJ. Laser-Raman spectroscopy of living cells. Phys Reports. 1980;60(4):201–24.
[15] Volkov SN, Kosevich AM. Conformation oscillations of DNA. Mol Biol (Mosk). 1987;21(3):797-806.
[16] Volkov SN, Kosevich AM, Wainreb GE. Theoretical studies in low-frequency vibrations of DNA macromolecules. Biopolym. Cell. 1989; 5(6):32-9.
[17] Chou KC. Low-frequency collective motion in biomacromolecules and its biological functions. Biophys Chem. 1988;30(1):3-48.
[18] Chou KC, Mao B. Collective motion in DNA and its role in drug intercalation. Biopolymers. 1988;27(11):1795-815.
[19] Lord RC, Thomas GJ. Raman spectral studies of nucleic acids and related molecules—I Ribonucleic acid derivatives. Spectrochim Acta A. 1967;23(9):2551–91.
[20] Lautie A, Novak A. Spectres infrarouges et Raman de l'adenine, de son sel de sodium et de quelques derives deuteries. J Chim Phys. 1974; 71(3):415-20.
[21] Savoie R, Jutier J-J, Prizant L, Beauchamp AL. Raman and infrared spectra of methylmercury complexes of adenine. Spectrochim Acta A. 1982;38(5):561–8.
[22] Delabar J-M, Majoube M. Infrared and Raman spectroscopic study of 15N and D-substituted guanines. Spectrochim Acta A. 1978;34(2):129–40.
[23] Mathlouthi M, Seuvre AM, Koenig JL. F.t.-i.r. and laser-Raman spectra of guanine and guanosine. Carbohydr Res. 1986;146(1):15-27.
[24] Susi H, Ard JS, Purcell JM. Vibrational spectra of nucleic acid constituents—II. Spectrochim Acta A. 1973;29(4):725–33.
[25] Susi H, Ard JS. Planar valence force constants and assignments for pyrimidine derivatives. Spectrochim Acta A. 1974;30(9):1843–53.
[26] Mathlouthi M, Seuvre AM, Koenig JL. F.t.-i.r. and laser-Raman spectra of cytosine and cytidine. Carbohydr Res. 1986;146(1):1-13.
[27] Susi H, Ard JS. Vibrational spectra of nucleic acid constituents—I. Planar vibrations of uracil. Spectrochim Acta A. 1971;27(9):1549–62.
[28] Maleev VYa, Ctanevich AYe. The absorption spectra of nitrogenous bases crystals in the far infrared. Spectroscopy in solids. Leningrad, Nauka, 1969; 4:95-100.
[29] Beetz CP, Ascarelli G. The low frequency vibrations of pyrimidine and purine bases. Spectrochim Acta A. 1980;36(3):299–313.
[30] Shimanouchi T, Harada I. Far-Infrared Spectra of Cyanuric Acid, Uracil, and Diketopiperazine. J Chem Phys. 1964;41(9):2651-5.
[31] Hirakawa AY, Okada H, Sasagawa S, Tsuboi M. Infrared and Raman spectra of adenine and its 15N and 13C substitution products. Spectrochim Acta A. 1985;41(1-2):209–16.
[32] Govorun DN, Zheltovskiy NV. Hardware complex for the study of Raman spectra of biopolymers. Abstracts of reports 6th Conf. on spectroscopy of biopolymers. Kharkiv, 1988; 98-99.
[33] Bobrov AV, Muldakhmetov ZhM. Raman spectroscopy. Alma-Ata, Nauka, 1981; 153 p.
[34] Kalinkina IN, Grechushnikov BN. Decomposition methods for the complex spectral contour into individual components. Spectroscopy of Molecules and Crystals: Abstracts of 5th respublikanckoy shkoly-seminara. Kyiv, Naukova Dumka, 1983; 193-9.
[35] Tanabe K, Hiraishi J. Correction of finite slit width effects on Raman line widths. Spectrochim Acta A. 1980;36(4):341–4.
[36] Tret'yak SM, Mitkevich VV, Sukhodub LF. Adenine structure, its alkyl derivatives and their crystalline hydrates. Abstracts of reports 7th All-Union. symp. Pushchino, 1986; 125-6.
[37] Harada I, Lord RC. Low-frequency infrared and Raman spectra of some adenine and uracil crystals. Spectrochim Acta A. 1970;26(12):2305–18.
[38] Babkov LM, Puchkovskaya GA, Makarenko SP, Gavrilko TA. IR spectroscopy of molecular hydrogen-bonded crystals. Kyiv, Naukova Dumka, 1989; 160 p.