Biopolym. Cell. 1990; 6(2):100-104, 113.
Gene-Engineering Biotechnology
Plasmid transformation of Streptococcus lactis sphaeroplasts.
1Rumer L. M., 1Livshlts V. A.
  1. All-Union Research Institute of Genetics and Breeding of Industrial Microorganism, Glavmicrobioprom at Council of Ministers of the USSR
    Moscow, USSR


The optimal conditions for production of transformable spheroplasts of Streptococcus lactis using lysozyme were determined. It has been established that viability of Streptococcus lactis cells fell rapidly when concentration of lysozyme or duration of lysozyme treatment increased. The successful transformation of Streptococcus lactis spheroplasts by two heterolo-gous plasmids pRT84 and pLF2 was performed. It was found that expression of Bacillus amylollquefaciens gene coding for amylase cloned in pRT84 took place in Streptococcus lactis cells.


[1] Kondo JK, McKay LL. Transformation of Streptococcus lactis Protoplasts by Plasmid DNA. Appl Environ Microbiol. 1982;43(5):1213-5.
[2] Kondo JK, McKay LL. Mutanolysin for Improved Lysis and Rapid Protoplast Formation in Dairy Streptococci. J Dairy Sci. 1982;65(8):1428–31.
[3] Okamoto T, Fujita Y, Irie R. Fusion of protoplasts of streptococcus lactis. Agric Biol Chem. 1983;47(11):2675–6.
[4] Okamoto T, Fujita Y, Irie R. Protoplast formation and regeneration of Streptococcus lactis cells. Agric Biol Chem. 1983;47(2):259–63.
[5] Kondo JK, McKay LL. Plasmid transformation of Streptococcus lactis protoplasts: optimization and use in molecular cloning. Appl Environ Microbiol. 1984;48(2):252-9.
[6] Yu RST, Kyle WSA, Hung TV, Azad AA. Aspects of genetic transformation involving protoplasts and purified lac plasmid of Streptococcus lactis. Milchwissenschaft. 1984; 39(8): 476-479
[7] Simon D, Rouault A, Chopin MC. High-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA. Appl Environ Microbiol. 1986;52(2):394-5.
[8] Kok J, van der Vossen JM, Venema G. Construction of plasmid cloning vectors for lactic streptococci which also replicate in Bacillus subtilis and Escherichia coli. Appl Environ Microbiol. 1984;48(4):726-31.
[9] Gasson MJ. Production, regeneration and fusion of protoplasts in lactic streptococci. FEMS Microbiology Lett. 1980;9(2):99–102.
[10] Woskow SA, Kondo JK. Effect of Proteolytic Enzymes on Transfection and Transformation of Streptococcus lactis Protoplasts. Appl Environ Microbiol. 1987;53(10):2583-7.
[11] Sanders ME, Nicholson MA. A method for genetic transformation of nonprotoplasted Streptococcus lactis. Appl Environ Microbiol. 1987;53(8):1730-6.
[12] Malke H. Genetics of resistance to macrolide antibiotics and lincomycin in natural isolates of Streptococcus pyogenes. Mol Gen Genet. 1974;135(4):349-67.
[13] Avakov AS, Chernovskaia TV, Bidnenko VE, Abalakina EG, Iomantas IuV. Interferon secreted by Bacillus subtilis is retained by membranes. Dokl Akad Nauk SSSR. 1986;288(3):717-20.
[14] Torban EB, Yomantas YuV, Livshits VA. Studing a new plasmid from Lactobacillus fermentum. Biology, biotechnology and cultivation of microorganisms. Materials of IV Repub scientific and practical conference. Tashkent 1989:82.
[15] Terzaghi BE, Sandine WE. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975;29(6):807-13.
[16] Thomas TD, Jarvis BD, Skipper NA. Localization of proteinase(s) near the cell surface of Streptococcus lactis. J Bacteriol. 1974;118(2):329-33.
[17] Chang S, Cohen SN. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA. Mol Gen Genet. 1979;168(1):111-5. PubMed PMID: 107388.
[18] Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979;7(6):1513-23.
[19] Kang Y, Kim JH, Ryu DDY. Protoplast fusion of Lactobacillus casei. Agric Biol Chem. 1987;51(8):2221–7.