Biopolym. Cell. 1990; 6(2):91-100.
Gene-Engineering Biotechnology
Construction of hybrid lacZ genes to study the E. coli rpljl operon genes expression mechanisms
1Kroupskaya I. V., 1Zhyvoloup A. N., 1Paton E. B.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


Using the pNM481 plasmid vector 5'-terminal fragments of rplJ and rplL genes were fused in-frame to gene lacZ. Hybrid p-alactosidases activity, encoded by genes rplJ'— lacZ and rplL'-lacZ was compared. In contrast to rplL'-lacZ, extension of rplJ fused portion resulted in the decrease of the p-gal activity level. Different efficiency of translations seems to be the most probable mechanism, providing the excess synthesis of r-protein L7/L12 in vivo.


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