Biopolym. Cell. 1990; 6(2):81-87.
Structure and Function of Biopolymers
Elastic scattering spectroscopy of yersinia pestis capsular protein solutions
1Khebtsou N. G., 1Nikiforov V. V., 1Melnikov A. G., 2Merkulova T. K., 2Serdobintsev L. N.
  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Academy of Sciences of the USSR
    Saratov, USSR
  2. All-Union Institute "Microbe"
    Saratov, USSR

Abstract

The theory of «scattering spectrum» method is developed which permits size and concentration of particles or macromolecules to be determined in dilute solutions. Experimental results from studies of Yersinia restis capsular protein solusions by the «scatt-ering spectrum» method are compared with viscosimetric and sedimentation measurement data. Alternative variants for possible shape and structure of capsular protein macromolecules under physiological conditions are discussed.

References

[1] Bennett LG, Tornabene TG. Characterization of the antigenic subunits of the envelope protein of Yersinia pestis. J Bacteriol. 1974;117(1):48-55.
[2] Veynblat VI, Nikiforov VV, Kormilitsin AV. Hydrodynamic characterization of the capsular antigen of plague activator. Questions of the genetics, molecular biology and microbiology of plague and cholera. Saratov, inst. "Microbe" press, 1985; 37-42.
[3] Anisimov PI, Serdobintsev LN, Ivanov IuV, Taranenko TM, Naumov AV. Various physico-chemical properties of the Yersinia pestis capsular protein. Mol Gen Mikrobiol Virusol. 1987;(2):24-7. Russian.
[4] Cantor CR, Schimmel PR. Biophysical Chemistry (3 volumes). San Francisco, WH Freeman and Co., 1980 (Part I: The Conformation of Biological Macromolecules. Part II: Techniques for the Study of Biological Structure and Function. Part III: The Behavior of Biological Macromolecules, 1369 p.)
[5] Dynamic light scattering. Applications of photon correlation spectroscopy. Ed. R. Pecora. New York; London: Plenum press, 1985. 420 p.
[6] Dobychin PD, Lomakin AV, Mevkh NG, Noskin VA, Balabonov SM. Investigation of polydisperse actin solutions by quasi-elastic light scattering. Biopolym Cell. 1986; 2(1):23-9.
[7] Noskin VA, Shmelev GE, Lomakin AV, Petrova-Mastakova LG, Parfenova NS, Belova EV, Klimov AN. High density lipoprotein conformation changes in the process of saturation by cholesterol. Biopolym Cell. 1986; 2(6):293-301.
[8] Lozovski? VT, Shmelev GE, Noskin VA, Lapshin EN, Dobretsov GE. Size distribution of plasma lipoproteins. Biofizika. 1987;32(2):285-91. Russian.
[9] Klenin VI, Khlebtsov NG, Severinov AV, Lebedev LG. Determination of the parameters of supramolecular structures in dilute solutions of polymers by the scattering spectrum. Vysokomolekulyarnye soedineniya. 1987; 20(9):2136-41.
[10] Bhatnagar HL, Heller W. Theoretical Investigations on the Light Scattering of Spheres. XIV. Wavelength Exponent of Differential Scattering Spectra for an Angle of Observation of 90°. J Chem Phys. 1964;40(2):480-3.
[11] Boren K, Hafman D. Absorption and Scattering of Light by Small Particles. Moscow, Mir, 1986; 660 p.
[12] Klenin VI, Shcheglov SYu, Lavrushin VI. Characteristic functions of disperse systems scattering. Saratov: Sarat. Univ. press, 1977; 176 p. Khlebtsov NG
[13] Kerker M. The scattering of light and other electromagnetic radiations. New York; London: Acad, press, 1969. 660 p.
[14] Eskin VE. Light Scattering by Polymer Solutions. Leningrad, Nauka, 1986; 288 p.
[15] Khlebtsov NG. Scattering matrix for the anisotropic ellipsoids, comparable to the wavelength of light. Optika i spektroskopiia. 1979; 46(2):341-5.
[16] Dubichev AG, Serdobintsev LN, Vorontsov ED, et al. Investigation of the processes of dissociation-association of the capsular antigen of the plague microbe. Immunology and specific prevention of especially dangerous infections. Saratov, inst. "Microbe" press, 1986; 99-105.
[17] Tanford C. Physical chemistry of macromolecules. New York, Wiley, 1961; 710 p.