Transfer of human preproinsulin gene into streptozotocin diabetic rats

Titok, T. G.; Kostetsky, I. E.; Chaikovskaya, T. L.; Kochubej, T. P.; Zharova, L. G.; Kirilenko, S. D.; Melnik, I. M.; Silvanskaya, E. M.; Buzhievskaya, T. I.; Kordium, V. A.

doi:10.7124/bc.00025F

Biopolym. Cell. 1990; 6(2):76-80.

http://dx.doi.org/10.7124/bc.00025F

Transfer of human preproinsulin gene into streptozotocin diabetic rats

1Titok T. G., 1Kostetsky I. E., 2Chaikovskaya T. L., 1Kochubej T. P., 1Zharova L. G., 1Kirilenko S. D., 2Melnik I. M., 2Silvanskaya E. M., 1Buzhievskaya T. I., 1Kordium V. A.

Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
Kiev, USSR
Institute of Endocrinology and Metabolism, Ministry of Public Health of the Ukrainian SSR
Kiev, USSR

Abstract

Recombinans plasmid encapsulated in liposomes containing DNA sequences of human preproinsulin gene (pAINS) was injected into the liver of streptozotocin diabetic rats. The injection of pAINS decreased glycemia level and increased immunoreactive blood product concentration. Maximal effect was detected 6—8 h after injection, and insulin concentration returned to their initial levels after injection.

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References

[1] Laub O, Rutter WJ. Expression of the human insulin gene and cDNA in a heterologous mammalian system. J Biol Chem. 1983;258(10):6043-50.

[2] Walther R, Leibiger I, Kiessling U, Sarrach D, Zühlke H. Transfer of a human preproinsulin gene containing plasmid into non-pancreatic mammalian cells. Biomed Biochim Acta. 1988;47(4-5):343-8.

[3] Fromont-Racine M, Bucchini D, Desbois P, Pictet R, Jami J. Human insulin gene in transgenic mouse lines. Biomed Biochim Acta. 1988;47(4-5):349-53.

[4] Keller G, Paige C, Gilboa E, Wagner EF. Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent haematopoietic precursors. Nature. 1985 Nov 14-20;318(6042):149-54.

[5] Garver RI Jr, Chytil A, Courtney M, Crystal RG. Clonal gene therapy: transplanted mouse fibroblast clones express human alpha 1-antitrypsin gene in vivo. Science. 1987;237(4816):762-4.

[6] Nicolau C, Le Pape A, Soriano P, Fargette F, Juhel MF. In vivo expression of rat insulin after intravenous administration of the liposome-entrapped gene for rat insulin I. Proc Natl Acad Sci U S A. 1983;80(4):1068-72.

[7] Anderson WF. Prospects for human gene therapy in the born and unborn patient. Clin Obstet Gynecol. 1986;29(3):586-94.

[8] Joyce C. Americans plan gene therapy on people. New Sci. 1988. 119(1624):24.

[9] Neznanov NS, Troianovskiĭ BM, Gartel' AL, Makarova IV, Bendukidze KA. Molecular cloning of the human insulin gene. Mol Gen Mikrobiol Virusol. 1987;(7):14-5.

[10] Zapadnyuk IP, Zapadnyuk VI, Zakhariya EA, Zapadnyuk BV. Laboratory animals. Breeding, maintenance, use in the experiment. Kyiv, Vyshcha shkola, 1983; 243 p.

[11] Lyashenko VA, Drozhennikov VA, Molotkovskaya IM. Mechanisms of immune cells activation. Moscow, Medicine, 1988; 239 p.

[12] King GL, Kaher CR. Effect of insulin on growth in vivo and cell in culture. In: Control of animal cells proliferation. Eds. A. L. Boyton, H. L. Leffert. New York : Acad, press, 1985. Vol. 1: 201-49.

[13] Kosaki A, Kuzuya H, Yoshimasa Y, Yamada K, Okamoto M, Nishimura H, Kakehi T, Takeda J, Seino Y, Imura H. Regulation of glucose-transporter gene expression by insulin in cultured human fibroblasts. Diabetes. 1988;37(11):1583-6.