Biopolym. Cell. 1990; 6(2):65-71.
Population and family analysis of polymorphism of DNA sequences linked to the cystic fibrosis gene
1Voronina O. V., 1Gaitskhoki V. S., 2Ivashchenko T. E., 2Baranov V. S., 2Gorbunova V. N., 1Kalinin V. M., 3Livshitz L. A., 2, 4Venozhinskas M. T.
  1. Institute of Experimental Medicine, Academy of Medical Sciences of the USSR
    Leningrad, USSR
  2. Institute of Obstetrics and Gynecology, Academy of Medical Sciences of the USSR
    Leningrad, USSR
  3. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR
  4. Institute for Protection of Mothers and Children, Ministry of Public Health of the Lithuanian SSR
    Vilnius, USSR

Abstract

The allelic polymorphism of human DNA loci linked to cystic fibrosis gene (D7S8, Met and D7S23) was studied using blot hybridization and polymerase chain reaction in the populations of healthy donors in Leningrad and in donors from Moscow (D7S8) and Kiev (Met). The restriction fragment length polymorphism (RELP) in Leningrad and Kiev populations corresponds to RELP found in Northern Europe countries while in Moscow population a shift in the allele frequencies is observed, that appears to depend upon the contribution of mongoloids having very low frequency of cystic fibrosis. The marked, linkage disequilibrium between cystic fibrosis mutation and particular RELP alleles in D7S23 locus (KM.19 and XV-2c probes) is found in the affected families.

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