Biopolym. Cell. 1990; 6(2):31-35.
A model for genetic correction of family hypercholesterolemia
1Dolzhanskaya N. B., 1Mandelshtam M. Yu., 1Patkin E. L., 1Kuznetsov A. S., 2Vikhanskaya F. L., 2Krutilina R. I., 1Gaitskhoki V. S.
  1. Institute of Experimental Medicine, Academy of Medical Sciences of the USSR
    Leningrad, USSR
  2. Institute of Cytology, Academy of Sciences of the USSR
    Leningrad, USSR


The expression of human low-density lipoprotein receptor (rLDL) was studied in mouse fibroblasts (1-929 line) transformed by the pMSVL recombinant plasmid. This plasmid contains full-length rLDL cDNA and hybrid promoter composed of regulatory elements from early SV40 promoter and glucocorticoid-responsive enhancer of Molony sarcoma virus. The expression of rLDL c DNA in transformed cells was demonstrated using dot and blot RNA-cDNA hybridization as well as immunofluorescent analysis.


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