Biopolym. Cell. 1990; 6(1):100-103.
http://dx.doi.org/10.7124/bc.000250
Short Communications
Comparison of efficiency of use of early and late promoters of T7 phage for β-galactosidase genie expression in Escherichia coli
1Glushakova L. G., 1Romanovskaya O. R., 1Kordium V. A.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

The model system for comparison of the efficiency of use of early and late promoters of T7 phage for the β-galactosidase gene expression has been constructed. The following strategy of construction has been chosen. The gene of T7 phage RNA-polymerase has been inserted into DNA of phage cloning vector. The model gene z of lac operon of E. coli (EcoRI-Sall digest of pLZ56) has been inserted into DNA of plasmid GEM-1 (firm «Promega») which contains phage T7 late promoter. The cloned pLZ56 fragment of DNA contains also S-D sites and A3 promoter specifically recognized by E. coli RNA-polymerase. Some features of b-galactosidase synthesis during transcription of the z gene under the control of the late promoter of phage T7 and under the control of A3 promoter are shown.
Full text: (PDF, in Russian)

References

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