Biopolym. Cell. 1990; 6(1):63-66.
Comparative studies in efficiency of expression of bacterial β-galactosidase in vivo by the immunofluorescence method
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
Kiev, USSR
Abstract
The expression of some vector molecules encoding the synthesis of bacterial p-galactosidase in mouse liver hepatocytes 24 h after direct injection has been investigated. The advantages of immunofluorescence method with the microcytophotometry for rapid test of gene expression at the cell level are discussed.
Full text: (PDF, in Russian)
References
[1]
Coons AH, Leduc EH, Connolly JM. Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J Exp Med. 1955;102(1):49-60.
[2]
An G, Hidaka K, Siminovitch L. Expression of bacterial beta-galactosidase in animal cells. Mol Cell Biol. 1982;2(12):1628-32.
[3]
O'Hare K, Benoist C, Breathnach R. Transformation of mouse fibroblasts to methotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reductase. Proc Natl Acad Sci U S A. 1981;78(3):1527-31.
[4]
Kostetsky IE, Shpilevaya SP, Likhacheva LI, Zharova LC, Irodov DM, Kirillova VS, Kordium VA. Expression of Escherichia coli?-Galactosidase in murine hepatocytes. Biopolym Cell. 1989; 5(2):51-8.
[5]
Rhodes JM, Blom J. Cytochemical localization of beta-galactosidase in resident and inflammatory peritoneal macrophages from C57BL mice. Histochemistry. 1986;86(2):159-64.
[6]
Zilber A. Immunological analysis. Moscow, Medicine, 1968; 185 p.
[7]
Ernst B, Tasman D. Immunology. Immunological Methods. Moscow, Mir, 1979; 373-413.