Biopolym. Cell. 1989; 5(6):101-104.
http://dx.doi.org/10.7124/bc.0000FF
Short Communications
The effect of Escherichia coli ribosomal protein S12 mutations on the efficiency of deoxyribonucleotide matrix poly(dT) translation
1Groisman I. S., 1Potapov A. P.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

The efficiency of poly(dT) translation has been studied in cell-free systems from wild-type E. coli and streptomycin-resistant mutants with altered ribosome protein S12. The data show that there is a positive correlation between poly(U) misreading and efficiency of poly(dT) translation. Mutant ribosomes translate poly (U)-template more accurately than ribosomes from wild-type bacteria and they are less efficient in translation of poly(dT) as well. The ribosome seems to select codon-anticodon pair as a whole unit. The data are in good agreement with hypothesis of stereospecific stabilization of codon-anticodon complex by the ribosome.
Full text: (PDF, in Russian)

References

[1] Potapov AP. A stereospecific mechanism for the aminoacyl-tRNA selection at the ribosome. FEBS Lett. 1982;146(1):5-8.
[2] Potapov AP. Mechanism of the stereospecific stabilization of codon-anticodon complexes in ribosomes during translation. Zh Obshch Biol. 1985;46(1):63-77.
[3] McCarthy BJ, Holland JJ. Denatured DNA as a direct template for in vitro protein synthesis. Proc Natl Acad Sci U S A. 1965;54(3):880-6.
[4] Salas J, Bollum FJ. Biosynthesis polydeoxynucleotides as direct templates for polypeptide synthesis. J Biol Chem. 1968;243(5):1012-5.
[5] Potapov AP, Soldatkin KA, Soldatkin AP, El'skaya AV. Comparative study of Poly(U) and Poly(dT) template activity in cell-free protein synthesizing systems from Escherichia coli and wheat germ. Biopolym. Cell. 1988; 4(3):133-8.
[6] Potapov AP, Soldatkin KA, Soldatkin AP, El'skaya AV. The role of a template sugar-phosphate backbone in the ribosomal decoding mechanism. Comparative study of poly(U) and poly(dT) template activity. J Mol Biol. 1988;203(4):885-93.
[7] Davies J, Gilbert W, Gorini L. Streptomycin, suppression, and the code. Proc Natl Acad Sci U S A. 1964;51:883-90.
[8] Miller JH. Experiments in molecular genetics. Cold Spring Harbor Laboratory, 1972, 466 p.
[9] Soldatkin AP, Zheltovskaia NI, Obcharenko GV, El'skaia AV. Ambiguous polyuridylic acid translation by transport RNA from various eukaryotic substances. Ukr Biokhim Zh. 1983;55(6):603-7.