Biopolym. Cell. 1989; 5(6):25-32.
http://dx.doi.org/10.7124/bc.0000F3
Structure and Function of Biopolymers
Effect of alkali-earth and transition metal ions on hydrodynamic and macromolecular parameters of DNA
1Kornilova S. V., 1Shkorbatov A. G., 1Blagoi Yu. P.
  1. Institute for Low Temperature Physics and Engineering, Academy of Sciences of the Ukrainian SSR
    Kharkov, USSR

Abstract

DNA intrinsic viscosity [η] and the excluded volume parameter e iare stated to depend on the concentrations and the binding degrees of Mg2+, Mn2+, Ga2+, Cu2+ ions with different NaCl contents (2 ·10-4+10-1 M). The substantial changes of the [η] value and the parameter e in the presence of small contents of bivalent metal ions are shown to be due to the ion binding to the phosphate 'groups and nitrogen bases of DNA. Bivalent ion (Me2+)-DNA binding results in the suppression of long-range effects in a macromolecule with concentrations by some orders of magnitude lower than those in the case of monovalent ions. The effect of transition metal ions (Mn2+, Cu2+) on the value and the excluded volume parameter is greater than that of the alkali-earth (Ca2+, Mg2+) ones due to their binding not only to DNA phosphate groups but aJso to the nitrogen bases even with small concentrations (5·10–6 M). The intrinsic viscosity and the excluded volume parameter are shown to depend universally on the binding degree of bivalent ions.
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