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Structure and Function of Biopolymers
Isolation of seryl-tRNA synthetase from Thermus thermophilus HB-27.
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
A method to isolate seryl-tRNA synthetase from Thermus thermophilus is described. It includes ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparine-sepharose and hydrophobic chromatography on poliyvinyl sorbent Toyopearl HW-65. The yield of highly purified enzyme was 4 mg from 1 kg of T. thermophilus cells. Seryl-tRNA synthetase is a dimer protein (α2 type) with molecular mass of 90 kDa.
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