Biopolym. Cell. 1989; 5(5):83-86.
Structure and Function of Biopolymers
Isolation of seryl-tRNA synthetase from Thermus thermophilus HB-27.
1Yaremchuk A. D., 1Tukalo M. A., 1Konovalenko A. V., 1Egorova S. P., 1Matsuka G. Kh.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


A method to isolate seryl-tRNA synthetase from Thermus thermophilus is described. It includes ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparine-sepharose and hydrophobic chromatography on poliyvinyl sorbent Toyopearl HW-65. The yield of highly purified enzyme was 4 mg from 1 kg of T. thermophilus cells. Seryl-tRNA synthetase is a dimer protein (α2 type) with molecular mass of 90 kDa.


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