Biopolym. Cell. 1989; 5(5):70-75.
Structure and Function of Biopolymers
Studies in correlation between poly(U) misreading and efficiency of poly(dT) translation in the cell-free protein-synthesizing systems of E. coli
1Croisman I. S., 1Potapov A. P.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR


The efficiency of poly(dT) translation and misreading of poly(U) has been studied in cell-free systems from wild-type E. coli and streptomycin-resistant mutants with altered ribosome protein S12. The data show that there is a positive correlation between poly(U) misreading and efficiency of poly(dT) translation. Conditions promoting misreading of poly(U): an increase in the concentration of neomycin, kanamycin, streptomycin antibiotics, of Mg2+ ions stimulate poly(dT) translation as well. A series of antibiotics activities in promotion of both poly(U) misreading and poly(dT) translation is the same: neo-, mycin> kanamycin > streptomycin. The more accurate ribosomes with altered protein S12 are less efficient in translation of poly(dT). T he data obtained are in good agreement with the hypothesis on stereospecific stabilization of codon-anticodon complexes on the ribosome.


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