Biopolym. Cell. 1989; 5(4):103-106.
Short Communications
Peculiarities of protein synthesis on endogenous mRNAs in cell-free systems from germs of the different maize forms
1Marchenko M. M., 1Oplachko L. T., 1Kostyshin S. S., 1Yazlouitskaya L. S.
  1. State University of Chernovtsy
    Chernovtsy, USSR

Abstract

The activity of polypeptide synthesis on the endogenous mRNAs and poly(U) in the cell-free system from germs of the different maize forms is studied. Translational activity on the endogenous mRNAs in the cell-free system isolated from the hybrid maize germs is established to increase. The polypeptide synthesis stimulation by poly(U) in parental forms of super heterosis hybrids is higher than in generation F1.

References

[1] Oplachko LT, Kostyshin SS, Yakovleva LA, Marchenko MM. Activity of ribosomes of hybrid and inbred maize seedlings in a cell-free protein synthesis system. Fiziologiia rasteniy. 1985; 32(4):710-4.
[2] Erkeev MI, Kudoyarova GR. Study of the activity of protein-synthesizing system in heterotic hybrids of maize. Fiziologiia rasteniy. 1981; 28(4):880-2.
[3] Onarev VG. Biochemistry and molecular genetics of heterosis. Heterosis. Minsk: Nauka i tekhnika, 1982; 163-178.
[4] Yamada M, Ishige T, Ohkawa Y.Reappraisal of Ashby's hypothesis on heterosis of physiological traits in maize, Zea mays L. Euphytica. 1985. 34(3):593-8.
[5] Yamada M. Heterosis in embryo of maise, Zea mays L. Bull Nat Inst Agrobiol Res. 1985; 1:85-98.
[6] Roberts BE, Paterson BM. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973;70(8):2330-4.
[7] Marcus A, Efron D, Weeks DP. The wheat embryo cell-free system. Methods Enzymol. 1974;30:749-54.
[8] Mans RJ, Novelli GD. Measurement of the incorporation of radioactive amino acids into protein by a filter-paper disk method. Arch Biochem Biophys. 1961;94(1):48–53.
[9] Kostyshin SS, Khlus LN, OPlachko LT, Marchenko MM. RNA and Protein synthesis in the early Period of germination of corn germ and connection with heterosis. Sel'skokhozyaystvennaya biologiya. 1988; 1:35-8.