Biopolym. Cell. 1989; 5(3):95-101.
http://dx.doi.org/10.7124/bc.0000C9
Structure and Function of Biopolymers
Monoclonal antibodies for one-step isolation of highly purified urokinase
1Kraiasyuk G. A., 1Jakubov L. Z., 1Sinitsyn V. V., 1Domogatsky S. P., 1Rohklin O. V., 3Koltsova S. V., 2Bynyaeva N. A., 2Fedorova Z. D., 3Samsonov G. V.
  1. Research Institute of Experimental Cardiology, National Cardiological Research Centre, Academy of Medical Sciences of the USSR
    Moscow, USSR
  2. Institute of Haematology and Blood Transfusion, Ministry of Public Health of the RSFSR
    Leningrad, USSR
  3. Institute of High-Molecular Weight Compounds, Academy of Sciences of the USSR
    Leningrad, USSR

Abstract

Five independent hybridom clones producing monoclonal antibodies with high affinity and specificity for human urokinase are isolated using specially adopted screening technique. Both inhibiting urokinase activity and noninhibiting antibodies are obtained. They do not crossreact with other serin proteases and some of them have dissociation constants lower than 10 pmol/1. Immunosorbents with these monoclonal antibodies are effective for isolation of urokinase from urine: 2 mg of antibodies attached to 1 ml of sepharose binds 0.5 mg of urokinase.
Full text: (PDF, in Russian)

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