Biopolym. Cell. 1989; 5(3):77-79.
http://dx.doi.org/10.7124/bc.0000C5
Structure and Function of Biopolymers
Isolation and crystallization of ribosomal protein TS9
1Agalarov S. Ch., 1Eliseikina I. A.
  1. Institute of Protein Research, Academy of Sciences of the USSR
    Pushchino, Moscow Region, USSR

Abstract

A method is suggested to isolate ribosomal protein TS9 from SOS subparticles of ribosomes from extremal-thermophilic bacterium Thermus thermophilus. Rhomb-shaped crystals of this protein with maximal dimensions of 0.5 mm have been obtained. A method of vapour diffusion in a «hanging» drop with 2-methyl-2.4-pentanediol (MPD) as a precipitant has been used for crystallization.
Full text: (PDF, in Russian)

References

[1] Appelt K, Dijk J, Reinhardt R, Sanhuesa S, White SW, Wilson KS, Yonath A. The crystallization of ribosomal proteins from the 50 S subunit of the Escherichia coli and Bacillus stearothermophilus ribosome. J Biol Chem. 1981;256(22):11787-90.
[2] Appelt K, White SW, Wilson KS. Proteins of the Bacillus stearothermophilus ribosome. Crystallization of proteins L30 and S5. J Biol Chem. 1983;258(21):13328-30.
[3] Liljas A, Newcomer ME. Purification and crystallization of protein complex from Bacillus stearothermophilus ribosomes. J Mol Biol. 1981;153(2):393-8.
[4] Sedelnikova SE. Isolation of individual Proteins from 50S subParticles of Thermus thermoPhilus ribosomes. crystallization of TL7 Protein. BioPolym. Cell. 1987; 3(3):163-6.
[5] Wilson KS, Appelt K, Badger J, Tanaka I, White SW. Crystal structure of a prokaryotic ribosomal protein. Proc Natl Acad Sci U S A. 1986;83(19):7251-5.
[6] Yusupov MM, Trakhanov SD, Barynin VV, Borovyagin VL, Garber MB, Sedelnikova SE, Selivanova OM, Tischenko SV, Shirokov VA, Edintsov YN. Crystallization of the 30S subParticles of Thermus-thermophilus subparticles. Dokl Akad Nauk SSSR. 1987; 292(5):1271-4.
[7] Gogia ZV, Yusupov MM, SPirina TN. Structure of Thermus thermophilus ribosomes. Method of isolation and Purification of the ribosomes. Mol Biol (Mosk). 1986;20(2):519-26.
[8] Kenny JW, Lambert JM, Traut RR. Cross-linking of ribosomes using 2-iminothiolane (methyl 4-mercaptobutyrimidate) and identification of cross-linked proteins by diagonal polyacrylamide/sodium dodecyl sulfate gel electrophoresis. Methods Enzymol. 1979;59:534-50.
[9] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.