Selective fluorescent labelling of the RNA ends to study its macromolecule structure in the solution

Bakin, A. V.; Shotsky, I. N.

doi:10.7124/bc.0000C4

Biopolym. Cell. 1989; 5(3):71-76.

http://dx.doi.org/10.7124/bc.0000C4

Structure and Function of Biopolymers

Selective fluorescent labelling of the RNA ends to study its macromolecule structure in the solution

1Bakin A. V., 1Shotsky I. N.

M. V. Lomonosov State University
Moscow, USSR

Abstract

The method por selective fluorescent labelling of the polynucleotides ends is described. Reactions are performed in water with high yields (90–100 %) followed by no modification of the RNA bases. The distances between the ends of oligouridylates of different length in duplex of poly(U)-poly(A) is determined by the singlet-singlet energy transfer method.

Full text: (PDF, in Russian)

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