Biopolym. Cell. 1989; 5(3):54-60.
http://dx.doi.org/10.7124/bc.0000C1
Structure and Function of Biopolymers
rRNA-protein interactions in Escherichia coli 70S ribosomes and in complex of initiation by data of cross-links with bifunctional Pt(II) derivative
1Chistyakov P. G., 1Abdukayumov M. A., 1Venijaminova A. G., 1Vladimirov S. N., 1Graifer D. M., 1Kazakov S. A., 1Karpova G. G.
  1. Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR

Abstract

rRNA-protein cross-links in 70S 35S-labelled ribosomes in the free state and within initiation complex ribosome-AUGU6-fMet-tRNAfMet were investigated using bifunctional cleavable reagent {dichloro [N,N,N',N'-tetrakis- (2-aminoethyl) -1.6-hexamethylendiamine-diplatinum(II)]}dichloride. The sets of proteins cross-linked to 16S and 23S rRNA in free 70S ribosomes and in 70S initiation complex were shown to slightly differ. It was found that one of L7/L12 dimers borders on one of 16S rRNA sites (a distance <5 nm) both in free ribosomes and in initiation complex.
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