Biopolym. Cell. 1989; 5(3):49-53.
Structure and Function of Biopolymers
Affinity modification of chromatin with an alkylating hexadecadeoxyribothymidylate derivative
- Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
Novosibirsk, USSR
Abstract
Alkylating derivative of hexadecadeoxyribothymidylate bearing 4-(N-2-chloroethyl-N-methylamino) benzylamine residue on its 5'-terminal phosphate was used for affinity modification of complementary poly (A) sequences in chromatin DNA. The DNA reactivity was not influenced by transcription and replication processes. DNA in suspension chromatin fraction and in intact nuclei was more readily alkylated as compared to that from the soluble chromatin fraction. This effect was attributed to higher superhelicity of DNA in intact nuclear DNA-protein structures. Protein-free isolated DNA was by several orders of magnitude less reactive than the chromatin DNA. Reaction of alkylating oligonucleotide derivatives with DNA is the sequence specific process, since it is suppressed by excess of an arbitrary hexadecanucleotide.
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