Biopolym. Cell. 1989; 5(2):102-104.
http://dx.doi.org/10.7124/bc.0000B0
Short Communications
Isolation of Threonyl–tRNA synthetase from Thermos thermophilus
1Yaremchuk A. D., 1Tukalo M. A., 1Egorova S. P., 1Matsuka G. Kh.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

A method for isolation of high-purified threonyl-tRNA synthetase from T. thermophllus is described, including ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparine-sepharose and hydrophobic chromatography on Toyopearl HW-65. Yield of the purified enzyme was 9 mg from 1 kg of T. thermophllus cells. The enzyme is a dimer protein (a2 type) with molecular mass of 148 kDa.
Full text: (PDF, in Russian)

References

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