Biopolym. Cell. 1989; 5(2):102-104.
Short Communications
Isolation of Threonyl–tRNA synthetase from Thermos thermophilus
1Yaremchuk A. D., 1Tukalo M. A., 1Egorova S. P., 1Matsuka G. Kh.
- Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
Kiev, USSR
Abstract
A method for isolation of high-purified threonyl-tRNA synthetase from T. thermophllus is described, including ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparine-sepharose and hydrophobic chromatography on Toyopearl HW-65. Yield of the purified enzyme was 9 mg from 1 kg of T. thermophllus cells. The enzyme is a dimer protein (a2 type) with molecular mass of 148 kDa.
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