Biopolym. Cell. 1989; 5(2):69-73, 106.
http://dx.doi.org/10.7124/bc.0000A9
Structure and Function of Biopolymers
Changes in functional properties of restriction enzymes under the influence of (2'-5')Oligoadenylates in vitro
1Tkachuk Z. Yu., 1Tkachuk L. V., 2Kvasyuk E. I., 2Zaitseva G. V., 2Kalinichenko E. N., 2Mikhailopulo I. A., 1Matsuka G. Kh.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR
  2. Institute of Bioorganic Chemistry, Academy of Sciences of the Byelorussian SSR
    Minsk, USSR

Abstract

It is found that «core» trimers of (2'-5') oligoadenylic acid, viz., (A2'p)2A and-(3'dA2'p)23'dA, exert an influence on functional properties of restriction enzymes EcoRl, BamHl, and Hindlll. The presence of (2'-5') trimers in the reaction mixture containing DNA and the restriction enzyme results in (i) an inhibition of the restriction reaction, (ii) site-specific restriction of DNA λ, or (iii) nonspecific hydrolysis of DNA λ. In the last case restriction enzymes function as nonspecific DNAses.
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References

[1] Torrence PF. Molecular foundations of interferon action. Molecular Aspects of Medicine. 1982;5(3):129–71.
[2] Sen GC. Mechanism of interferon action: progress toward its understanding. Prog Nucleic Acid Res Mol Biol. 1982;27:105-56.
[3] Lengyel P. Biochemistry of interferons and their actions. Annu Rev Biochem. 1982;51:251-82.
[4] Knight M, Cayley PJ, Silverman RH, Wreschner DH, Gilbert CS, Brown RE, Kerr IM. Radioimmune, radiobinding and HPLC analysis of 2-5A and related oligonucleotides from intact cells. Nature. 1980;288(5787):189-92.
[5] Doetsch PW, Suhadolnik RJ, Sawada Y, Mosca JD, Flick MB, Reichenbach NL, Dang AQ, Wu JM, Charubala R, Pfleiderer W, Henderson EE. Core (2'-5')oligoadenylate and the cordycepin analog: inhibitors of Epstein--Barr virus-induced transformation of human lymphocytes in the absence of interferon. Proc Natl Acad Sci U S A. 1981;78(11):6699-703.
[6] Eppstein DA, March YV, Schryver BB, Larsen MA, Barnett JW, Verheyden JP, Prisbe EJ. Analogs of (A2'p)nA. Correlation of structure of analogs of ppp(A2'p)2A and (A2'p)2A with stability and biological activity. J Biol Chem. 1982;257(22):13390-7.
[7] Eppstein DA, Schryver BB, Marsh YV, Larsen MA, Kurahara CG. Dephosphorylated Core of 2'-5' Oligoadenylate Exerts Its Antimitogenic Effect through Mechanisms Different from the 2'-5'A-Dependent Endonuclease. Journal of Interferon Research. 1983;3(3):305–11.
[8] Black PL, Henderson EE, Pfleiderer W, Charubala R, Suhadolnik RJ. 2',5'-Oligoadenylate trimer core and the cordycepin analog augment the tumoricidal activity of human natural killer cells. J Immunol. 1984;133(5):2773-7.
[9] Suhadolnik RJ, Doetsch PW, Devash Y, Henderson EE, Charubala R, Pfleiderer W. 2′,5′-Adenylate and Cordycepin Trimer Cores: Metabolic Stability and Evidence for Antimitogenesis without 5′-Rephosphorylation. Nucleosides and Nucleotides. 1983;2(4):351–66.
[10] Eppstein DA, Marsh YV, Schryver BB. Mechanism of antiviral activity of (XyloA2'p)2XyloA. Virology. 1983;131(2):341-54.
[11] Torrence PF, Imai J, Lesiak K, Jamoulle J-C, Sawai H, Warinnier J, et al. Strategies in the Design of Oligonucleotides as Potential Antiviral Agents. Targets for the Design of Antiviral Agents. 1984;259–85.
[12] Johnston M, Torrence PF. The role of interferon-induced proteins, double-stranded RNA and 2', 5'-oligoadenylate in the interfcron-mediafed inhibition of viral translation. Interferon: mechanisms of production and action. Ed. R. M Friedman - Amsterdam: Elsevier, 1984. Vol. 3:189-298
[13] Kaiser AD, Hogness DS. The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg. J Mol Biol. 1960;2:392-415.
[14] Kyasyuk EI, Kulak TI, Khripach NB, Mikhailopulo IA, Uhlmann E, Charubala R, et al. Nucleotides XXIV; 1 Preparative Synthesis of Trimeric (2'-5') Oligoadenylic Acid . Synthesis. 1987;1987(06):535–41.
[15] Kvasyuk EI, Kulak TI, Kalinitchenko EN, Podkopaeva TL, Mikhailopulo IA, Pfleiderer W. Chemical synthesis of some (2'-5')oligonucleotides, analogues of (2'-5')oligoadenylic acid. Russian Journal of Bioorganic Chemistry. 1985; 11(9):1227-38.
[16] Sharp PA, Sugden B, Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973;12(16):3055-63.
[17] Maniatis T, Fritsch EF, Sambrook J. Molecular cloning - a laboratory manual. New York, Cold Spring Harbor, 1982; 545 p.