Biopolym. Cell. 1989; 5(2):58-61.
http://dx.doi.org/10.7124/bc.0000A7
Discussions
Studies in possibility of expression of exogenic human insulin gene in cultured mammalian fibroblasts
1Neborachko L. N., 1Lukash L. L., 1Troyanovsky B. M., 1Varzanova I. S., 1Buzhievskaya T. I., 1Kordium V. A.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Expression of human insulin genomic gene is observed in human and murine fibroblasts. Protein-product (proinsulin or insulin) detected by ELISA is secreted by fibroblasts. The protein-product secreted level achieves its peak on 9th-10th days and may be expressed in 125-160 ng/ml of culture medium. The similar dependence of proinsulin / insulin secretion by human and murine fibroblasts is determined.
Full text: (PDF, in Russian)

References

[1] Jones G. Biotechnology is set to improve patient care. Pract . Biotechnology. 1986; 7(11):2.
[2] Cordell B, Goodman M. Evolution of the mammalian insulin gene. Genes and tumor genes. New York Raven press, 1982:115-119.
[3] Blundell TL, Humbel RE. Hormone families: pancreatic hormones and homologous growth factors. Nature. 1980;287(5785):781-7.
[4] de Haen C, Swanson E, Teller DC. The evolutionary origin of proinsulin. Amino acid sequence homology with the trypsin-related serine proteases detected and evaluated by new statistical methods. J Mol Biol. 1976;106(3):639-61.
[5] Birch NP, Christie DL, Renwick AG. Proinsulin-like material in mouse foetal brain cell cultures. FEBS Lett. 1984;168(2):299-302.
[6] Narang SA, Brousseau R, Georges F, Michniewicz J, Prefontaine G, Stawinski J, Sung W. The human preproinsulin gene: synthesis, cloning, gene modification, and expression studies. Can J Biochem Cell Biol. 1984;62(4):209-16.
[7] Niven MJ, Hitman GA. The molecular genetics of diabetes mellitus. Biosci Rep. 1986;6(6):501-12. ttp://
[8] Laub O, Rall L, Bell GI, Rutter WJ. Expression of the human insulin gene in an alternate mammalian cell and in cell extracts. J Biol Chem. 1983;258(10):6037-42.
[9] Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell. 1981;23(1):175-82.
[10] Laub O, Rutter WJ. Expression of the human insulin gene and cDNA in a heterologous mammalian system. J Biol Chem. 1983;258(10):6043-50.
[11] Itoh N, Okamoto H. Translational control of proinsulin synthesis by glucose. Nature. 1980;283(5742):100-2.
[12] Cordell B, Diamond D, Smith S, Pünter J, Schöne HH, Goodman HM. Disproportionate expression of the two nonallelic rat insulin genes in a pancreatic tumor is due to translational control. Cell. 1982;31(3 Pt 2):531-42.
[13] Kakita K, Giddings S, Permutt MA. Biosynthesis of rat insulins I and II: evidence for differential expression of the two genes. Proc Natl Acad Sci U S A. 1982;79(9):2803-7.
[14] Nielsen DA, Chou J, MacKrell AJ, Casadaban MJ, Steiner DF. Expression of a preproinsulin-beta-galactosidase gene fusion in mammalian cells. Proc Natl Acad Sci U S A. 1983;80(17):5198-202.
[15] Neznanov NS, Troianovskii BM, Gartel AL, Makarova IV, Bendukidze KA. Molecular cloning of the human insulin gene. Mol Gen Mikrobiol Virusol. 1987;(7):14-5.
[16] Reznikoff CA, Brankow DW, Heidelberger C. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 1973;33(12):3231-8.
[17] Graham FL, van der Eb AJ. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973;52(2):456-67.
[18] van Wyke KL, Hinshaw VS, Bean WJ Jr, Webster RG. Antigenic variation of influenza A virus nucleoprotein detected with monoclonal antibodies. J Virol. 1980;35(1):24-30.