Biopolym. Cell. 1989; 5(1):89-93.
http://dx.doi.org/10.7124/bc.00009A
Short Communications
Is it possible to graft an «alien» active site on a protein globule?
1Finkelstein A. V.
  1. Institute of Protein Research, Academy of Sciences of the USSR
    Pushchino, Moscow Region, USSR

Abstract

If a protein globule serves only as a rigid «foundation» for the active site, then the other «alien» active site can be «grafted» onto it by site-directed mutagenesis. Under this hypothesis, the mutations are singled out which should be introduced to the T4 phage lysozyme to «graft» it the protease activity.
Full text: (PDF, in Russian)

References

[1] Fersht A. Enzyme structure and mechanism. New York, WH Freeman, 1977.
[2] Marquart M, Walter J, Deisenhofer J, Bode W, Huber R. The geometry of the reactive site and of the peptide groups in trypsin, trypsinogen and its complexes with inhibitors. Acta Cryst B. 1983;39(4):480–90.
[3] Drenth J, Hol WG, Jansonius JN, Koekoek R. A comparison of the three-dimensional structures of subtilisin BPN' and subtilisin novo. Cold Spring Harb Symp Quant Biol. 1972;36:107-16.
[4] Bryan P, Pantoliano MW, Quill SG, Hsiao HY, Poulos T. Site-directed mutagenesis and the role of the oxyanion hole in subtilisin. Proc Natl Acad Sci U S A. 1986;83(11):3743-5.
[5] Ptitsyn OB. Protein as an edited random copolymer. Mol Biol (Mosk). 1984;18(3):574-90.
[6] Bliumenfeld LA. Problems of Biological Physics. Moscow, Nauka, 1977; 336 p.
[7] Leatherbarrow RJ, Fersht AR. Protein engineering. Protein Eng. 1986;1(1):7-16.
[8] Finkelshtein AV, Kirpichnikov P, Ptitsyn OB, Skryabin KG. Construction of the protein molecules. Vestn akad nauk sssr. 1988; 1:102-111.
[9] Bernstein FC, Koetzle TF, Williams GJ, Meyer EF Jr, Brice MD, Rodgers JR, Kennard O, Shimanouchi T, Tasumi M. The Protein Data Bank. A computer-based archival file for macromolecular structures. Eur J Biochem. 1977;80(2):319-24.
[10] Bolin JT, Filman DJ, Matthews DA, Hamlin RC, Kraut J. Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 A resolution. I. General features and binding of methotrexate. J Biol Chem. 1982;257(22):13650-62.
[11] Remington SJ, Eyck LF, Matthews BW. Atomic coordinates for T4 phage lysozyme. Biochem Biophys Res Commun. 1977;75(2):265-70.