Biopolym. Cell. 1989; 5(1):66-72.
Gene-Engineering Biotechnology
Restriction mapping of chloroplast DNAs of five Nicotiana species
1Komarnitsky I. K., 1Samoylov A. M., 1Gleba Yu. Yu.
  1. N. G. Kholodny Institute of Botany, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Restriction mappings for five Nicotiana species of australian origin — N. eastii, N. occidentalis, N. exigua and american one — N. undulata, N. chinensis have been constructed using Sal Gl, Xho I, Pst 1, Pvu II and Sma I restriction endonucleases. Most of changes found in chloroplast DNA are positioned in the central part of the large single-copy region and are adjacent to inverted repeats. These changes are, probably, due to deletions and/or insertions. On the contrary, the changes in the small single-copy region are mainly caused by point mutations.

References

[1] Seyer P, Kowallik KV, Herrmann RG. A physical map of Nicotiana tabacum plastid DNA including the location of structural genes for ribosomal RNAs and the large subunit of ribulose bisphosphate carboxylase/oxygenase. Curr Genet. 1981;3(3):189-204.
[2] Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M. The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression. EMBO J. 1986;5(9):2043-2049.
[3] Kung SD, Zhu YS, Shen GF. Nicotiana chloroplast genome III. Chloroplast DNA evolution. Theor Appl Genet. 1982;61(1):73-9.
[4] Shen GF, Chen K, Wu M, Kung SD. Nicotiana chloroplast genome. IV. N accuminata Has lager invert repeats and genome size. Mol Gen Genet. 1982;187(1):12–8.
[5] Zhu YS, Duvall EJ, Lovett PS, Kung SD. Nicotiana chloroplast genome. V Construction, mapping and expression of clone library of N. otophora chloroplast DNA. Mol Gen Genet. 1982;187(1):61–6.
[6] Salts Y, Herrmann RG, Peleg N, Lavi U, Izhar S, Frankel R, Beckmann JS. Physical mapping of plastid DNA variation among eleven Nicotiana species. Theor Appl Genet. 1984;69(1):1-14.
[7] Tassopulu D, Kung SD. Nicotiana chloroplast genome : 6. Deletion and hot spot - a proposed origin of the inverted repeats. Theor Appl Genet. 1984;67(2-3):185-93.
[8] Kolodner R, Tewari KK. The molecular size and conformation of the chloroplast DNA from higher plants. Biochim Biophys Acta. 1975;402(3):372-90.
[9] Bookjans G, Stummann BM, Henningsen KW. Preparation of chloroplast DNA from pea plastids isolated in a medium of high ionic strength. Anal Biochem. 1984;141(1):244-7.
[10] Jurgenson JE, Bourque DP. Mapping of rRNA genes in an inverted repeat in Nicotiana tabacum chloroplast DNA. Nucleic Acids Res. 1980;8(16):3505-16.
[11] Fluhr R, Edelman M. Physical mapping of Nicotiana tabacum chloroplast DNA. Mol Gen Genet.1981;181(4):484–90.
[12] Hildebrand M, Jurgenson JE, Ramage RT, Bourque DP. Derivation of a physical map of chloroplast DNA from Nicotiana tabacum by two-dimensional gel and computer-aided restriction analysis. Plasmid. 1985;14(1):64-79.
[13] Komarnitskiy IK, Gleba YuYu, Grebenkin AP. Chloroplast DNA as a marker in the gene systematics of the genus Nicotiana. Plant Genome: Proc. of reports of sci. conf. Chernivtsi, Czernowitz. Univ. Press, 1983; 37.
[14] Albertini AM, Hofer M, Calos MP, Miller JH. On the formation of spontaneous deletions: the importance of short sequence homologies in the generation of large deletions. Cell. 1982;29(2):319-28.
[15] Calos MP, Miller JH. Transposable elements. Cell. 1980;20(3):579-95.
[16] Chiu WL, Sears BB. Recombination between chloroplast DNAs does not occur in sexual crosses of Oenothera. Mol Gen Genet. 1985;198(3):525-8.
[17] Metzlaff M, B?rner T, Hagemann R. Variations of chloroplast DNAs in the genus Pelargonium and their biparental inheritance. Theor Appl Genet. 1981;60(1):37-41.
[18] De Haas JM, Boot KJM, Haring MA, Kool AJ, Nijkamp HJJ. A Petunia hybrida chloroplast DNA region, close to one of the inverted repeats, shows sequence homology with the Euglena gracilis chloroplast DNA region that carries the putative replication origin. Mol Gen Genet. 1986;202(1):48–54.
[19] Koller B, Delius H. Origin of replication in chloroplast DNA of Euglena gracilis located close to the region of variable size. EMBO J. 1982;1(8):995-8.
[20] Schlunegger B, Stutz E. The Euglena gracilis chloroplast genome: structural features of a DNA region possibly carrying the single origin of DNA replication. Curr Genet. 1984;8(8):629-34.
[21] Ravel-Chapuis P, Heizmann P, Nigon V. Electron microscopic localization of the replication origin of Euglena gracilis chloroplast DNA. Nature. 1982;300(5887):78–81.
[22] Palmer JD, Thompson WF. Chloroplast DNA rearrangements are more frequent when a large inverted repeat sequence is lost. Cell. 1982;29(2):537-50.
[23] Teeri TH, Saura A, Lokki J. Insertion polymorphism in pea chloroplast DNA. Theor Appl Genet. 1985;69(5-6):567-70.
[24] Myers AM, Grant DM, Rabert DK, Harris EH, Boynton JE, Gillham NW. Mutants of Chlamydomonas reinhardtii with physical alterations in their chloroplast DNA. Plasmid. 1982;7(2):133-51.
[25] Kolodner R, Tewari KK. Inverted repeats in chloroplast DNA from higher plants. Proc Natl Acad Sci U S A. 1979;76(1):41-5.
[26] Psareva EN. On the genus Nicotiana. Proc. All-Union Res. Inst. for tobacco and shag. 1963; Is. 153:10-153.