Biopolym. Cell. 1989; 5(1):35-40.
http://dx.doi.org/10.7124/bc.000044
Structure and Function of Biopolymers
Photoaffinity modification of E-site of Escherichia coli ribosomes
1Vladimirov S. N., 1Graifer D. M., 1Zenkova M. A., 1Karpova G. Y., 1Olenina L. V., 2Kirillov S. V., 2Makarov E. M., 2Makhno V. I., 2Semenkov Yu. P.
  1. Institute of Bioorganic Chemistry, Siberian Branch of the Academy of Sciences of the USSR
    Novosibirsk, USSR
  2. B. P. Konstantinov Institute of Nuclear Physics, Academy of Sciences of the USSR
    Gatchina, Leningrad distr., USSR

Abstract

Photoaffinity modification of E. coli ribosomes with tRNAPhe derivative bearing arylazidogroups statistically scattered over guanine residues was studied under conditions of binding of the deacylated tRNAphe (tRNAPhe) derivative at 70S ribosomal E-site (without template, in the presence of antibiotic edeine). Proteins S8, S15, S17, S18, S21 and L10 were found to be labelled. Another set of proteins — L2, L7/L12, L10, L16, L25 and L26 — was identified as being labelled within the complex of the tRNAPhe derivative with isolated SOS subunits.
Full text: (PDF, in Russian)

References

[1] Rheinberger HJ, Sternbach H, Nierhaus KH. Three tRNA binding sites on Escherichia coli ribosomes. Proc Natl Acad Sci U S A. 1981;78(9):5310-4.
[2] Grajevskaja RA, Ivanov YV, Saminsky EM. 70-S ribosomes of Escherichia coli have an additional site for deacylated tRNA binding. Eur J Biochem. 1982;128(1):47-52.
[3] Kirillov SV, Makarov EM, Semenkov YuP. Quantitative study of interaction of deacylated tRNA with Escherichia coli ribosomes. Role of 50 S subunits in formation of the E site. FEBS Lett. 1983;157(1):91-4.
[4] Lill R, Robertson JM, Wintermeyer W. tRNA binding sites of ribosomes from Escherichia coli. Biochemistry. 1984;23(26):6710-7.
[5] Rheinberger HJ, Nierhaus KH. Testing an alternative model for the ribosomal peptide elongation cycle. Proc Natl Acad Sci U S A. 1983;80(14):4213-7.
[6] Rheinberger HJ, Nierhaus KH. Allosteric interactions between the ribosomal transfer RNA-binding sites A and E. J Biol Chem. 1986;261(20):9133-9.
[7] Semenkov YuP, Makarov EM, Kirillov SV. Quantitative study of interaction of deacylated tRNA with the P, A and E sites of Escherichia coli ribosomes. Biopolym. Cell. 1985; 1(4):183-93.
[8] Kirillov SV, Makhno VI, Semenkov YP. Mechanism of codon-anticodon interaction in ribosomes. Direct functional evidence that isolated 30S subunits contain two codon-specific binding sites for transfer RNA. Nucleic Acids Res. 1980;8(1):183-96.
[9] Grajevskaja RA, Saminski EM, Bresler SE. Interaction of transfer RNA with ribosomes in the absence of messenger. Biochem Biophys Res Commun. 1972;46(3):1106-12.
[10] Parfenov DV, Saminskii EM. Deacylated tRNA binds with 50S subunits of Escherichia coli ribosomes at a special site not corresponding to the P'-site. Mol Biol (Mosk). 1985;19(5):1378-85.
[11] Bresler SE, Graevskaya RA, Ivanov YV, Saminskii EM. Interaction of transfer RNA with 50S ribosomal subunits of Escherichia coli in absence of templates. Mol Biol (Mosk). 1976;10(4):640-6.
[12] Babkina GT, Bausk EV, Graifer DM, Karpova GG, Matasova NB. The effect of aminoacyl- or peptidyl-tRNA at the A-site on the arrangement of deacylated tRNA at the ribosomal P-site. FEBS Lett. 1984;170(2):290-4.
[13] Vladimirov SN, Graifer DM, Karpova GG, Semenkov YuP, Makhno VI, Kirillov SV. The effect of GTP hydrolysis and transpeptidation on the arrangement of aminoacyl-tRNA at the A-site of Escherichia coli 70 S ribosomes. FEBS Lett. 1985;181(2):367-72.
[14] Semenkov IuP, Makhno VI, Makarov EM, Kirillov SV. The effect of the antibiotic edeine on tRNA interaction with A, P and E sites of Escherichia coli 70S ribosomes. Mol Biol (Mosk). 1984;18(5):1348-51.
[15] Vladimirov SN, Graifer DM, Karpova GG. The effect of poly(U) on the arrangement of tRNAPhe in donor tRNA-binding site of Escherichia coli ribosomes. FEBS Lett. 1982;144(2):332-6.
[16] Kirillov SV, Katunin VI, Semenkov YP. Mechanism of codon-anticodon interaction in ribosomes: comparative study of interaction of Phe-tRNAPhe and N-acetyl-Phe-tRNAPhe with the donor site of Escherichia coli ribosomes. FEBS Lett. 1981;125(1):15-9.