Biopolym. Cell. 2025; 41(3):191.
http://dx.doi.org/10.7124/bc.000B21
Molecular and Cell Biotechnologies
Development and comparison of two alanine aminotransferase-sensitive biosensors based on pyruvate oxidase and glutamate oxidase
1Mruga D. O., 1, 2Vakhovsky E. R., 1, 2Dzyadevych S. V., 1, 3Soldatkin O. O.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143
  2. Taras Shevchenko National University of Kyiv
    64, Volodymyrska Str., Kyiv, Ukraine, 01601
  3. Igor Sikorsky Kyiv Polytechnic Institute
    37, Beresteysky Ave., Kyiv, Ukraine, 03056

Abstract

Aim. To compare two developed biosensors based on pyruvate oxidase (POx) and glutamate oxidase (GlOx) for measuring alanine aminotransferase activity and to determine their main advantages and disadvantages. Methods. A three-electrode amperometric measurement scheme was used in the study. Platinum disk electrodes were used as working electrodes, and the signal was recorded using a PalmSens potentiostat. Immobilized POx and GlOx were used as recognition biomaterials. Results. The method and the optimal conditions for immobilization of each of the enzymes were selected. The procedure for creating the developed ALT-sensitive biosensors based on POx and GlOx was optimized. The optimal operating parameters for each sensor were chosen and analytical characteristics of both proposed biosensors were compared. POx biosensor has 1.5 times higher sensitivity, wider linear range and 2.5 lower detection limit, and unlike GlOx biosensor, can be used in a multibiosensor system for simultaneous measurement of ALT and AST. Conclusions. Taking into account the biosensor based on POx has more advantages to be used for ALT activity determination in the biological fluids.
Keywords: alanine aminotransferase, biosensor, amperometry, clinical significance, in vitro testing, kinetics
Full text: (PDF, in English)

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