Biopolym. Cell. 2025; 41(2):100.
Molecular and Cell Biotechnologies
Biotechnological production of chitosan: extraction and characterization from Trichoderma sp.
- Department of Biotechnology, Centre of Excellence for Microbial
and Plant Biotechnology, Jaypee Institute of Information Technology
A-10, Sector-62, Noida, Uttar Pradesh, India, 201309
Abstract
Aim. The study aimed at extraction and physicochemical characterization of fungal chitosan from Trichoderma reesei (MTCC 4876). Methods. Fungal chitosan was isolated using submerged fermentation over 4 days in three different growth media: Potato Dextrose Broth (PDB), Yeast Potato Dextrose Broth (YPDB), and Czapek Dox Broth (CDB). Growth kinetic parameters, including specific growth rate (µ) and doubling time (td), were determined for each medium. Chitosan extraction from the harvested biomass was performed using an alkaline treatment method. Physicochemical characterization of extracted chitosan using FTIR & DSC, UV-Vis spectroscopy for optical absorption properties and Photoluminescence (PL) spectroscopy to assess fluorescence behavior. Results. Among the tested media, PDB demonstrated the highest biomass yield of 116 mg/g. FTIR analysis confirmed the presence of functional groups analogous to commercial chitosan, indicating structural similarity. Thermal analysis via DSC validated the thermal stability of fungal chitosan. UV-Vis spectroscopy revealed significant optical absorption, while PL spectra exhibited notable fluorescence behavior, suggesting potential use in advanced material applications. Conclusions. In this study chitosan was successfully isolated from Trichoderma reesei (MTCC 4876), demonstrating distinct physicochemical properties comparable to the commercial chitosan. These findings highlight the potential of fungal chitosan for diverse industrial and commercial applications, particularly where optical and thermal properties are crucial.
Keywords: Trichoderma reesei, fungal chitosan, chitin, FTIR, DSC
Full text: (PDF, in English)
References
[1]
Rinaudo M. Chitin and chitosan: Properties and applications. Prog Polym Sci. 2023; 120:120987.
[2]
Straits Research. Chitosan Market Size, Share & Trends Analysis Report by Grade-2024. https://straitsresearch.com/report/chitosan-market
[3]
Kumar MNVR. A review of chitin and chitosan applications. Carbohydr Polym. 2022; 107687.
[4]
Dash M, Chiellini F, Ottenbrite RM, Chiellini E. Chitosan–a versatile semi–synthetic polymer in biomedical applications. Int J Biol Macromol. 2022; 108172.
[5]
Aranaz I, Harris R, Heras A. Chitosan chemistry and its functional properties. Polymer. 2022; 125678.
[6]
Jayakumar R, Prabaharan M, Sudheesh Kumar PT, Nair SV, Tamura H. Biomaterials based on chitin and chitosan in wound dressing applications. Biotechnol Adv. 2021; 107794.
[7]
Younes I, Rinaudo M. Chitin and chitosan preparation from marine sources. Colloids Surf B Biointerfaces. 2022; 112737.
[8]
Pillai CKS, Paul W, Sharma CP. Chitin and chitosan polymers: Chemistry, solubility and fiber formation. Enzyme Microb Technol. 2022; 110003.
[9]
Peter MG. Chitin and chitosan: Prospective industrial applications. Fungal Biol. 2021; 101005.
[10]
Dutta PK, Dutta J, Tripathi VS. Chitin and chitosan: Chemistry, properties and applications. Biochem Cell Biol. 2023; 106545.
[11]
Knaul JZ, Hudson SM, Creber KA. Improved mechanical properties of chitosan films. Food Chem. 2023; 135602.
[12]
Ravi Kumar MNV. Chitin and chitosan derivatives for biomedical applications. Chem Eng J. 2022; 139834.
[13]
Shahidi F, Abuzaytoun R. Chitin, chitosan, and co–products: Chemistry, production, applications, and health effects. Mar Pollut Bull. 2023; 115882.
[14]
Synowiecki J, Al–Khateeb NA. Production, properties, and some new applications of chitin and its derivatives. Bioresour Technol. 2023; 128496.
[15]
Domard A, Domard M. Chitosan and its derivatives. Surf Coat Technol. 2022; 128076.
[16]
Didecan D, Dumitriu S. Polysaccharides in medical applications. Food Bioprod Process. 2022; 101356.
[17]
Elsabee MZ, Abdou ES. Chitosan–based edible films and coatings. Int J Food Microbiol. 2022; 109857.
[18]
Khor E, Lim LY. Implantable applications of chitin and chitosan. Microbiol Res. 2022; 127157.
[19]
El Knidri H, Belaabed R, Addaou A, Laajeb A, Lahsini A. Extraction, chemical modification and characterization of chitin and chitosan. Enzyme Microb Technol. 2021; 109883.
[20]
Zhang J, He X, Wang Y, Zhao X. Production of cellulases by Trichoderma reesei. Biotechnol Rep. 2022; 006521.
[21]
Xu Y, Gallert C, Winter J. Biodegradation of chitosan. Mycol Res. 2023; 101230.
[22]
Ogawa K, Yui T, Miya M. Molecular dynamics simulation of chitosan and chitin. Synth Met. 2022; 116956.
[23]
Martin M, Meyer AS. Chitosan production by submerged fermentation. Biotechnol Rep. 2022; 101011.
[24]
Zhang L, Lin J, Han X. Optimization of chitosan extraction. LWT Food Sci Technol. 2022; 113455.
[25]
Pereira JF, Cavalcanti RC. Submerged fermentation for biopolymer production. Process Biochem. 2022; 108771.
[26]
Madigan MT, Martinko JM, Bender KS. Brock Biology of Microorganisms. 16th ed. Pearson; 2022.
[27]
Shuler ML, Kargi F. Bioprocess Engineering: Basic Concepts. 3rd ed. Prentice Hall; 2021.
[28]
Prescott LM, Harley JP, Klein DA. Microbiology. 11th ed. McGraw–Hill Education; 2023.
[29]
Pelczar MJ, Chan ECS, Krieg NR. Microbiology: Concepts and Applications. 2nd ed. McGraw–Hill; 2021
[30]
Dhillon GS, Kaur S, Brar SK, Verma M. Green synthesis approach: extraction of chitosan from fungus mycelia. Critical reviews in biotechnology. 2013; 33(4):379-403.
[31]
Moura A, Silva V, Pereira L. Determination of the degree of deacetylation of chitosan using infrared spectroscopy and NMR calibration. Carbohydr Polym. 2023; 312:120675.
[32]
Chen Y, Wang T, Li X. Comparative analysis of chitosan deacetylation methods: FTIR versus chemical titration. Int J Biol Macromol. 2022; 197:845-51.
[33]
Santos JP, Oliveira FJ, Costa AC. Improved FTIR–based method for accurate assessment of chitosan deacetylation. Polym Test. 2023; 115:107753.
[34]
Li H, Zhang Y, Chen J. Thermal characterization of chitosan: Effects on stability and application potential. Thermochim Acta. 2023; 725:179010
[35]
Patel R, Mehta P, Shah D. FT–IR spectroscopic analysis for characterization of chitosan from fungal and commercial sources. Spectrochim Acta A Mol Biomol Spectrosc. 2023; 287:122056.
[36]
Singh V, Kaur P, Verma R. Optical characterization of fungal and commercial chitosan using UV–Vis and photoluminescence spectroscopy. J Mol Struct. 2023; 1289:135678
[37]
Gachhi DB, Hungund BS. Two phase extraction, characterization and biological evaluation of chitin and chitosan from Rhizopus oryzae. J App Pharm Sci. 2018; 8(11):116-22
[38]
Zhao X, Wu Y, Chen Q. Photoluminescence properties of fungal–derived chitosan and its structural correlation. J Photochem Photobiol A Chem. 2023; 439:114482.
[39]
Kumar S, Meena R, Patel V. Comparative photoluminescence study of commercial and fungal chitosan for optical applications. Opt Mater. 2022; 134:113276.
[40]
Fernandes AC, Silva MJ, Costa RM. Infrared spectral interpretation of chitosan: Identification of characteristic functional groups. Vib Spectrosc. 2023; 117:103264.
