Biopolym. Cell. 2025; 41(1):52-62.
http://dx.doi.org/10.7124/bc.000B0E
Molecular Biomedicine
Non-invasive biomarkers for bladder cancer: a study on lncRNAs and DNA methylation
1Haviaz V. O., 1, 2Bratyshchenko A. S., 2Skrypnikova O. S., 1, 3Kariaka S. V., 1Honcharenko A. I., 1, 4Todoryshyn D. P., 1, 5Khokhliuk O. A., 1Rosohatska I. V., 1Kashuba V. I., 6Kononenko O. A., 7, 8Vikarchuk M. V., 1, 9Panasenko G. V., 1Mankovska O. S.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03143
  2. National University of Kyiv-Mohyla Academy
    2, Skovorody Str., Kyiv, Ukraine, 04070
  3. Institute of High Technologies, Taras Shevchenko National University of Kyiv
    4-G, Akademika Glushkova Ave., Kyiv, Ukraine, 03022
  4. Educational and Scientific Center "Institute of Biology and Medicine",
    Taras Shevchenko National University of Kyiv
    64/13, Volodymyrska Str., Kyiv, Ukraine, 01601
  5. International European University
    42Ð’, Akademika Hlushkova Str., Kyiv, Ukraine, 03187
  6. National Cancer Institute, Ministry of Health of Ukraine
    33/43, Yulia Zdanovskaya Str., Kyiv, Ukraine, 03022
  7. State Institution «Institute of Urology of NAMS of Ukraine»
    9-a, Yu. Kotsubyns'koho Str., Kyiv, Ukraine, 04053
  8. Military medical clinical center of Eastern Region
    63, Starokozats`ka Str., Dnipro, Ukraine, 49000
  9. XEMA LLC
    23, Akademika Yefremova Str., Kyiv, Ukraine, 03179

Abstract

Aim. To identify potential epigenetic markers for BC diagnosis and progression, including the methylation of gene promoters and the lncRNAs expression levels in tumor tissues and liquid biopsies. Methods. RT-qPCR, MSP. Results. Significant diagnostic value of the VIM, TMEFF2, NKX3.1, MYO3A, GDF15, and RASSF1A methylation in BC patients, increased the RASSF1A expression in tumor tissues and decreased the RASSF1A and ANRASSF1 in liquid biopsies. Correlations were identified between the studied markers and the clinical characteristics of patients. Conclusions. Methylation of the genes VIM, TMEFF2, NKX3.1, MYO3A, GDF15, and RASSF1A, and changes in the expression of the lncRNAs ANRASSF1, PANDAR, and GAS5 may play an important role in BC pathogenesis. The data obtained underscore the relevance of further studies on these markers for BC diagnosis and progression assessment.
Keywords: epigenetic biomarkers, DNA methylation, bladder cancer, lncRNAs, liquid biopsy, tumor tissues
Full text: (PDF, in English)

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