Aim. Hypericum perforatum L. and Gingko biloba L. are two examples of top-selling plants that have been cultivated by European and American phytopharmaceutical companies for many years [1]. Medicinal plant biotechnology could enhance the formation and accumulation of desirable natural products and develop new and effective pharmaceuticals. Hypericum perforatum L. extracts accumulate phytochemicals such as flavonoids, biflavones, phenolic acids, naphthodiantrone derivatives, and phloroglucinols. These extracts are known to have antidepressant, antidiabetic, antioxidant, antibacterial, antiviral, and anticancer activities [2]. The main aim of the present study was to investigate the antioxidant and antibacterial activities of Hypericum perforatum L. phytochemicals in callus culture extracts. Conclusions. The results suggest that the addition of the phytohormones TDZ (0.5 mg/L) and IAA (0.1 mg/L) to the MS medium is a suitable method to promote the formation of bioactive secondary metabolites in Hypericum perforatum L. callus cultures.