Aim. Lactic acid is a crucial human metabolite with numerous physiological roles and applications in various fields. It serves as a biomarker for conditions like hypoxia and kidney and liver issues and is used as a food additive and quality marker for food and beverages. Due to its broad usage, there is a demand for fast, reliable, and easy methods to analyze lactate. Biosensor measurement methods are emerging as they are quick, affordable, easy to use, and portable.This study aims to analyze existing biosensor systems for lactate determination and to adapt and optimize an amperometric biosensor based on lactate oxidase for measuring lactate in blood serum samples. Conclusions. The data obtained using the publication analysis parameters built into bibliographic systems were analyzed, and the directions for the development of biosensor methods of lactate determination were determined. The amperometric biosensor for measuring lactate concentration was optimized and adapted. Analysis of a number of blood serum samples using the proposed biosensor and a standard measurement method was performed and a high correlation of the results was shown.