Biopolym. Cell. 2024; 40(3):225-225.
Chronicle and Information
Isolation and characterization of extracellular vesicles from different types of mesenchymal stromal/stem cells for future translation research
- R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
45, Vasilkivska Str., Kyiv, Ukraine, 01022 - Biotechnology Laboratory, Medical Company «Good Cells»
9, Ivana Kramskoho Str., Kyiv, Ukraine, 03115 - Institute of Genetic and Regenerative Medicine, NAMS of Ukraine
67, Vyshhorodska Str., Kyiv, Ukraine, 04114 - M. D. Strazhesko National Scientific Center of Cardiology, Clinical and Regenerative Medicine, NAMS of Ukraine
5, Sviatoslava Khorobroho Str., Kyiv, Ukraine, 03151
Abstract
Aim. The aim of our study was to isolate and characterize EVs from umbilical cord-derived MSCs (UC-MSCs), adipose tissue-derived MSCs (ADSCs) and dental pulp stem cells (DPSCs). Conclusions. UC-MSCs and DPSCs are the most suitable cell types for large-scale EVs isolation. Based on size distribution and CD63/CD81 expression, EVs isolated from UC-MSCs, DPSCs, and ADSCs can be defined as exosomes. UC-MSC-derived EVs contain higher levels of growth factors and cytokines compared to DPSC- and ADSC-derived EVs, which may mediate stronger tissue regeneration through regulation of cell proliferation, immunosuppression, and vasculogenesis.
Keywords: extracellular vesicles, mesenchymal stromal/stem cells, exosomes, growth factors, cytokines
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