Biopolym. Cell. 2024; 40(3):208-208.
http://dx.doi.org/10.7124/bc.000AE8
Chronicle and Information
Predicting proteases integrated with membranes of gram-negative bacteria and their putative roles
1Kozyrovska N. O., 1Podolich O. V., 1Zaets I. Ye., 2Havryliuk O. A., 1Melnyk H. B., 1Zaika L. A., 1Zubova G. V.
  1. Institute of Molecular Biology and Genetics, NAS of Ukraine
    150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680
  2. D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine
    154, Academika Zabolotnoho Str., Kyiv, Ukraine, 03680

Abstract

Aim. Bacteria produce extracellular membrane vesicles (EV), which now are being investigated for their application as immunotherapeutics, vaccines, and drug delivery vehicles with promising early results [1, 2, 3]. The properties of EVs are harnessed for the development of next-generation microbe-inspired therapies. The immunologic effects as the main mechanism of EV actions is currently in preclinical development [4]. We are focused on another aspect of their putative application in translation medicine, namely, on enzymes associated with EVs from various bacterial species for cancer therapy. The present study aimed to identify and characterize Pseudomonas aeruginosa ATCC 10145 and Komagataeibacter obedience IMBG180 proteases associated with EVs and demonstrate their effect on in vitro human malignant cells. Conclusions. In silico proteogenomic analysis of the EV-associated proteases, along with functional studies, contribute to our understanding of the proteolytic activity of EVs and the potential applications of these nanostructures in biomedicine.
Keywords: nanovesicles, membrane–associatedproteases, apoptosis, cancer cells
Full text: (PDF, in English)

References

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